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  4. Rat matrix metalloproteinase 7,MMP-7 ELISA kit

Rat matrix metalloproteinase 7,MMP-7 ELISA kit

说明书下载
  • 中文名称：
    大鼠基质金属蛋白酶7(MMP-7)酶联免疫试剂盒
  • 货号：
    CSB-E07408r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠基质金属蛋白酶7（MMP-7）酶联免疫检测试剂盒（货号：CSB-E07408r），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的MMP-7含量，检测灵敏度可达15.6 pg/mL，线性范围为15.6-1000 pg/mL，适用于科研领域对MMP-7表达水平的精准分析。MMP-7作为基质金属蛋白酶家族成员，在细胞外基质降解、组织重塑及炎症反应等病理生理过程中发挥重要作用，尤其在纤维化疾病和肿瘤微环境调控研究中具有显著科研价值。本试剂盒提供标准品、预包被抗体及检测所需全套组分，操作流程优化后可在4小时内完成检测，未开封试剂盒在2-8℃条件下有效期为12个月，开封后建议1个月内使用完毕。适用于动物实验中对肝纤维化、肺损伤或肿瘤模型等疾病机制研究，帮助科研人员评估组织修复、炎症进展或肿瘤侵袭相关分子事件中MMP-7的动态变化，为体外实验提供可靠的定量检测方案。
  • 别名：
    Mmp7 ELISA Kit; Mmp-7Matrilysin ELISA Kit; EC 3.4.24.23 ELISA Kit; Matrin ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP-7 ELISA Kit; Pump-1 protease ELISA Kit; Uterine metalloproteinase ELISA Kit
  • 缩写：
    MMP7
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %88-98
    1:2Average %84
    Range %81-88
    1:4Average %90
    Range %85-94
    1:8Average %97
    Range %92-102
  • 回收率：
    The recovery of rat MMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-100
    EDTA plasma (n=4)9387-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.612 2.637 2.625 2.470
    5001.792 1.736 1.764 1.609
    2501.101 1.074 1.088 0.933
    1250.603 0.634 0.619 0.464
    62.50.431 0.414 0.423 0.268
    31.250.333 0.309 0.321 0.166
    15.60.232 0.219 0.226 0.071
    00.156 0.153 0.155 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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Mmp7 antibodies

Mmp7 Proteins

靶点详情

  • 功能：
    Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • 基因功能参考文献：
    1. Placental ischemia, possibly through the release of TNF-alpha, causes increases in the levels of matrix metalloproteinase (MMP)-1 and MMP-7, which could alter collagen deposition and cause inadequate uteroplacental and vascular remodeling in hypertension in pregnancy. PMID: 28626073
    2. Expression of MMP-7, c-Jun and c-Fos were increased in skin photoaging. PMID: 23870463
    3. Increased extracellular nerve growth factor precursor levels following seizures are stabilized by altered MMP-7 enzymatic activity, leading to increased neuronal death via activation of p75(NTR). PMID: 22238106
    4. MMP-7 significantly contributes to intestinal epithelial wound closure. IL-1beta increased MMP-7 levels beyond those seen during normal healing. PMID: 20655064
    5. important role of MMP-2, MMP-9, and TIMP-4 in hypertensive remodeling of the cortex and medulla in the SHR rat PMID: 12923405
    6. Adrenergic alpha-agonist-induced activation of matrix metalloproteinase-7 promotes vasoconstriction through the epidermal growth factor-receptor pathway. PMID: 14656925
    7. MMP-7, which was produced by osteoclasts at the tumor-bone interface, was capable of processing RANKL to a soluble form that promoted osteoclast activation. PMID: 15894268
    8. Diminished expression of MMP-7 may play a role in fibronectin accumulation in the diabetic kidney in response to advanced glycation end products and/or TGF-beta. PMID: 17554258
    9. Claudin-7, kidney injury molecule-1 (Kim-1), and matrix metalloproteinase-7 (MMP-7) changed by aging and changes attenuated by caloric restriction. PMID: 17670906
    10. These results suggest a novel role for noncanonical WNT signaling in maintaining kidney transplant tolerance with MMP7 being a key target. PMID: 18209025
    11. MMP-7 cleaves the NR1 subunit of the N-methyl-d-aspartate (NMDA) receptor to generate an N-terminal fragment of approximately 65 kDa PMID: 18644839
    12. Agonist signaling of both hypertension and hypertrophy depends on posttranscriptional and transcriptional mechanisms that involve MMP-7, which is transcriptionally connected with ADAM-12 PMID: 19398663

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：

    KEGG: rno:25335

    STRING: 10116.ENSRNOP00000014041

    UniGene: Rn.10282

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