Rat myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) ELISA kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清或血浆中髓过氧化物酶特异性抗中性粒细胞胞质抗体（MPO-ANCA）的酶联免疫试剂盒，基于双抗体夹心法原理设计，具有高灵敏度和特异性。MPO-ANCA是抗中性粒细胞胞质抗体的重要亚型，靶向髓过氧化物酶抗原，其异常表达与自身免疫性血管炎疾病密切相关，在显微镜下多血管炎、肉芽肿性多血管炎等疾病机制研究中具有重要价值。试剂盒检测范围为0.625-40 U/mL，可精准覆盖生理及病理浓度区间，标准品与样本均采用双复孔检测模式，配套提供预包被96孔板、检测抗体、HRP酶联物及显色底物等完整组分，操作流程约需3.5小时完成。样本类型兼容血清和血浆，试剂盒组分需于-20°C保存，有效期6个月。该产品适用于科研场景中血管炎动物模型的抗体水平动态监测、疾病机制探索以及药物干预效果评价等研究，为深入解析MPO-ANCA在免疫病理过程中的作用提供可靠工具。
别名：

N/A
缩写：

MPO-ANCA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

0.625 U/mL-40 U/mL
灵敏度：

0.156 U/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO-ANCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-95
1:2	Average %	97
	Range %	91-105
1:4	Average %	96
	Range %	92-100
1:8	Average %	93
	Range %	85-98

回收率：

The recovery of rat MPO-ANCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	91	84-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.