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  4. Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

文献引用 (17) 说明书下载
  • 中文名称：
    大鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒
  • 货号：
    CSB-E08788r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒（CSB-E08788r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NFκB p65含量。NFκB p65是重要的转录因子，在细胞的炎症、免疫、增殖和凋亡等多种生理病理过程中发挥关键作用。其激活机制多样，与多条信号通路相互关联。对其研究有助于深入了解疾病发病机制，为开发针对性疗法如抗炎、抗肿瘤药物等提供理论基础。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于基础科研中炎症机制研究、免疫调控实验或药物干预效果评估，例如在动物模型中分析NF-κB信号通路激活状态，或探究氧化应激、感染性疾病等实验条件下p65亚基的动态表达。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    NF-Kappa B,nuclear factor-kappa B
  • 缩写：
    NFκB p65
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %89
    Range %86-92
    1:200Average %99
    Range %97-103
    1:400Average %89
    Range %85-93
    1:800Average %95
    Range %91-99
  • 回收率：
    The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-97
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.039 2.022 2.031 1.887
    501.334 1.326 1.330 1.186
    250.908 0.902 0.905 0.761
    12.50.566 0.557 0.562 0.418
    6.250.358 0.364 0.361 0.217
    3.120.291 0.281 0.286 0.142
    1.560.243 0.232 0.238 0.094
    00.145 0.143 0.144 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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