Rat placenta growth factor,PLGF ELISA kit
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中文名称：
大鼠胎盘生长因子(PLGF)酶联免疫试剂盒
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货号：
CSB-E07400r
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规格：
96T/48T
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价格：
￥3600/￥2500
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其他：
产品详情
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产品描述：
CUSABIO定量检测大鼠胎盘生长因子（PLGF）的酶联免疫试剂盒（货号：CSB-E07400r），基于双抗体夹心原理设计，适用于分析血清、血浆及组织匀浆样本。PLGF作为血管内皮生长因子家族成员，在胎盘发育、血管生成及细胞增殖调控中起关键作用，其表达水平与妊娠相关病理生理过程密切相关。试剂盒采用特异性抗体对实现高灵敏度检测，线性范围为3.12-200 pg/mL，能够精确测定低丰度样本中的PLGF浓度。操作流程标准化，配套试剂完整，支持科研人员开展大鼠模型中妊娠并发症机制、组织修复调控或血管生成相关疾病研究。该试剂盒兼容多种生物样本类型，特别适用于探究胎盘发育异常、心血管疾病模型或肿瘤微环境中的PLGF动态变化，为体外实验提供可靠的定量分析工具。所有检测结果仅限科学研究使用，需严格遵循说明书操作规范以确保数据准确性。
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别名：
Pgf ELISA Kit; Plgf ELISA Kit; Placenta growth factor ELISA Kit; PlGF ELISA Kit
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缩写：
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Uniprot No.：
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种属：
Rattus norvegicus (Rat)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
3.12 pg/mL-200 pg/mL
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灵敏度：
0.78 pg/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Cancer
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PLGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-95 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-97 |
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| 1:8 |
Average % |
99 |
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| Range % |
93-102 |
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回收率：
| The recovery of rat PLGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-97 |
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| EDTA plasma (n=4) |
94 |
91-98 |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.246 |
2.234 |
2.240 |
2.085 |
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| 100 |
1.472 |
1.456 |
1.464 |
1.309 |
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| 50 |
0.901 |
0.914 |
0.908 |
0.753 |
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| 25 |
0.639 |
0.649 |
0.644 |
0.489 |
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| 12.5 |
0.364 |
0.389 |
0.377 |
0.222 |
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| 6.25 |
0.300 |
0.308 |
0.304 |
0.149 |
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| 3.12 |
0.252 |
0.242 |
0.247 |
0.092 |
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| 0 |
0.156 |
0.154 |
0.155 |
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本试剂盒所含材料：
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat PLGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled PLGF antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：
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货期：
3-5 working days
引用文献
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The involvement of long noncoding RNA APOA1-AS in the pathogenesis of preeclampsia
HY Yang,Human & experimental toxicology,2023
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Fructose Consumption Affects Placental Production of H2S: Impact on Preeclampsia-Related Parameters
X Zhao,Nutrients,2024
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Dendrobium nobile Lindley and its bibenzyl component moscatilin are able to protect retinal cells from ischemia/hypoxia by dowregulating placental growth factor and upregulating Norrie disease protein
Wen-Haur Chao.et al,BMC Complementary and Alternative Medicine,2018
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Celastrol attenuates symptoms of preeclampsia in rats by inhibiting matrix metalloproteinase-9
Sha Xiao.et al,European Journal of Pharmacology,2018
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Ferulic acid alleviates symptoms of preeclampsia in rats by upregulating vascular endothelial growth factor
Gong W.et al,Clin Exp Pharmacol Physiol. ,2017
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The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an l-NAME model of pre-eclampsia
Sooraj Baijnath.et al,Molecular and Cellular Biochemistry,2016
靶点详情
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功能：
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth.
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基因功能参考文献：
- Studied the influence of dendrobium nobile Lindley (DNL) extract and its bibenzyl component moscatilin on the expression of placental growth factor (PLGF) and Norrie disease protein (NDP) in hypoxia model in retinal cells. Found DNL and moscatilin are able to protect against retinal ischemic/hypoxic changes respectively by downregulating PLGF and upregulating NDP. PMID: 29933759
- this paper show that knockdown of PLGF mitigates damage in hyperoxic lung by blocking of NFkappaB signaling PMID: 27280587
- PLGF triggers focal adhesion disassembly in alveolar type II cells via inhibiting the activation of focal adhesion kinase. PMID: 25515701
- PlGF had a positive feedback regulation with 15-lipoxygenase expression and 15-hydroxyeicosatetraenoic acid generation. PMID: 24001991
- placental gene expression is modulated by gestational conditions that might disrupt the fetal growth PMID: 23691181
- PlGF repairs myocardial ischemia through mechanisms of angiogenesis, cardioprotection and recruitment of myo-angiogenic competent marrow progenitors PMID: 21969865
- augmentation of brain Placental growth factor is associated with development of epilepsy. PMID: 22079325
- PLGF expression in the lung tissue in rats with paraquat-induced pulmonary fibrosis is upregulated. PMID: 17997886
- This is the first demonstration that sustained intraocular PGF production induces vascular and retinal changes similar to those observed in the early stages of diabetic retinopathy. PMID: 21408222
- Intramyocardial injections of autologous myoblasts overexpressing placental growth factor improved cardiac function, attenuated adverse cardiac remodeling, induced angiogenesis, and probably enhanced survival of grafted myoblasts. PMID: 21329947
- This study shows for the first time the placental growth factor upregulation in regenerating livers, which is related to hypoxia stimulation and liver growth. PMID: 21088600
- Endothelial cell-released H2O2 acts as a positive regulator of placenta growth factor gene and protein expression in vascular smooth muscle cells. PMID: 21123739
- The ARPE19 human junctions in culture are modulated by VEGF through VEGFR-1 but not through VEGFR-2. PMID: 18421240
- The levels of PlGF-2 in non-malformed embryos of diabetic gestation and their placentas were significantly (P < 0.05) lower than the average of controls. PMID: 19048427
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亚细胞定位：
Secreted.
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蛋白家族：
PDGF/VEGF growth factor family
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数据库链接：
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