Rat platelet-derived growth factor-BB,PDGF-BB ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠血小板衍生生长因子BB（PDGF-BB）酶联免疫检测试剂盒（货号：CSB-E08924r），采用双抗体夹心ELISA技术，结合链霉亲和素-HRP信号放大系统，可定量检测血清、血浆、细胞培养上清液及组织匀浆等样本中的PDGF-BB含量。PDGF-BB作为血小板衍生生长因子家族的重要成员，在细胞增殖、血管生成及组织修复等过程中发挥关键作用，其表达水平与炎症反应、纤维化疾病等病理机制密切相关。本试剂盒检测范围覆盖39.063至2500 pg/mL的宽线性区间，可满足不同浓度样本的精准分析需求，实验流程包括样本预处理、抗体孵育、显色反应及吸光度测定，操作标准化且重复性良好。该产品适用于基础科研领域，如细胞模型中生长因子调控机制研究、动物疾病模型中PDGF-BB动态变化的定量分析，以及组织微环境修复相关实验的分子水平检测，为探索心血管疾病、组织再生及病理损伤等研究方向提供可靠工具。
缩写：

PDGF-BB
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

39.063 pg/mL-2500 pg/mL
灵敏度：

9.77 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	101-107
1:2	Average %	97
	Range %	94-100
1:4	Average %	88
	Range %	83-90
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	101-109
EDTA plasma (n=4)	110	107-113

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.