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中文名称:大鼠血小板衍生生长因子BB(PDGF-BB)酶联免疫试剂盒
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货号:CSB-E08924r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:CUSABIO大鼠血小板衍生生长因子BB(PDGF-BB)酶联免疫检测试剂盒(货号:CSB-E08924r),采用双抗体夹心ELISA技术,结合链霉亲和素-HRP信号放大系统,可定量检测血清、血浆、细胞培养上清液及组织匀浆等样本中的PDGF-BB含量。PDGF-BB作为血小板衍生生长因子家族的重要成员,在细胞增殖、血管生成及组织修复等过程中发挥关键作用,其表达水平与炎症反应、纤维化疾病等病理机制密切相关。本试剂盒检测范围覆盖39.063至2500 pg/mL的宽线性区间,可满足不同浓度样本的精准分析需求,实验流程包括样本预处理、抗体孵育、显色反应及吸光度测定,操作标准化且重复性良好。该产品适用于基础科研领域,如细胞模型中生长因子调控机制研究、动物疾病模型中PDGF-BB动态变化的定量分析,以及组织微环境修复相关实验的分子水平检测,为探索心血管疾病、组织再生及病理损伤等研究方向提供可靠工具。
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缩写:PDGF-BB
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, cell culture supernates, tissue homogenates
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检测范围:39.063 pg/mL-2500 pg/mL
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灵敏度:9.77 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cardiovascular
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 104 Range % 101-107 1:2 Average % 97 Range % 94-100 1:4 Average % 88 Range % 83-90 1:8 Average % 89 Range % 84-93 -
回收率:
The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 105 101-109 EDTA plasma (n=4) 110 107-113 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2500 2.794 2.753 2.774 2.578 1250 2.204 2.200 2.202 2.006 625 1.530 1.501 1.516 1.320 312.5 0.901 0.925 0.913 0.717 156.25 0.635 0.652 0.644 0.448 78.125 0.459 0.435 0.447 0.251 39.063 0.376 0.368 0.372 0.176 0 0.197 0.194 0.196 -
数据处理:
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货期:3-5 working days
引用文献
- Molecular mechanisms of interrod spacing-mediated osseointegration via modulating inflammatory response and osteogenic differentiation M Yu,Chemical Engineering Journal,2022
- PDGF-BB exhibited therapeutic effects on rat model of bisphosphonate-related osteonecrosis of the jaw by enhancing angiogenesis and osteogenesis Si-yong Gao, et al,Bone,2019
- Effects of local platelet-rich plasma injection on the rate of orthodontic tooth movement in a rat model: A histomorphometric study Ay?eg眉l G眉le莽.et al,American Journal of Orthodontics and Dentofacial Orthopedics,2017