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  4. Rat soluble cluster of differentiation 14,sCD14 ELISA Kit

Rat soluble cluster of differentiation 14,sCD14 ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    大鼠可溶性CD14(sCD14)酶联免疫试剂盒
  • 货号：
    CSB-E11178r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠可溶性CD14（sCD14）酶联免疫试剂盒（货号：CSB-E11178r），采用双抗体夹心法原理实现定量检测，适用于科研领域对大鼠血清、血浆及组织匀浆样本中sCD14蛋白水平的精准分析。CD14是先天免疫系统中的重要模式识别受体，其可溶性形式（sCD14）作为脂多糖/LBP复合物的循环受体，在介导革兰氏阴性菌感染引发的炎症反应中发挥关键作用，常被用于研究感染性疾病和炎症相关机制。该试剂盒检测灵敏度为28 ng/mL，线性范围覆盖28-1800 ng/mL，配备96孔预包被板、系列标准品、生物素标记检测抗体及全套实验试剂，通过显色反应实现目标蛋白的定量测定，适用于研究感染模型、炎症性疾病或免疫调控过程中sCD14的动态变化，为探索宿主免疫应答机制提供可靠工具。实验操作需配套酶标仪在450 nm波长下读数，建议通过标准曲线拟合进行浓度计算，实验全程需严格控制样本处理流程以保证数据准确性。
  • 别名：
    Cd14Monocyte differentiation antigen CD14 ELISA Kit; Myeloid cell-specific leucine-rich glycoprotein ELISA Kit; CD antigen CD14 ELISA Kit
  • 缩写：
    CD14
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    28 ng/mL-1800 ng/mL
  • 灵敏度：
    7 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %86-100
    1:2Average %99
    Range %90-103
    1:4Average %89
    Range %83-92
    1:8Average %95
    Range %84-98
  • 回收率：
    The recovery of rat sCD14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10294-108
    EDTA plasma (n=4)9689-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    18002.530 2.401 2.466 2.283
    9002.066 1.986 2.026 1.843
    4501.311 1.356 1.334 1.151
    2250.759 0.779 0.769 0.586
    112.50.456 0.478 0.467 0.284
    560.352 0.344 0.348 0.165
    280.271 0.263 0.267 0.084
    00.181 0.185 0.183
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Cd14 Proteins

靶点详情

  • 功能：
    Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).
  • 基因功能参考文献：
    1. This study shows that sCD14 impairs contractile function and insulin action in primary adult rat cardiomyocytes. PMID: 27816522
    2. CD 14 mRNA expression increased significantly after five days of bile duct ligation in liver, lung, spleen, and ileum. PMID: 25093541
    3. biliary drainage affects expression of inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats PMID: 23613625
    4. Burn injury-induced mitochondrial damage in cardiomyocytes was prevented in CD14 knockout mice. Burn injury produces CD14-dependent mitochondrial damage via oxidative stress in myocardium. PMID: 20348223
    5. Acute cerebral hemorrhage results in endotoxemia and widespread increases in CD14 and IL-1beta expression. PMID: 19534684
    6. Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia. PMID: 12046090
    7. Increase of CD14 mRNA expression may result in greater sensitivity to endotoxin and thus lead to liver injury. PMID: 12113681
    8. Although microglial-derived CD14 does not contribute to early events triggered by intrastriatal endotoxin treatment, it modulates the duration of inflammation within the cerebral tissue. PMID: 12218159
    9. Ethanol administration led to a significant synthesis of endotoxin receptor CD14 protein and its gene expression in Kupffer cells, which maybe result in the pathological changes of liver tissue and hepatic functional damages. PMID: 12632533
    10. the CD14 antigen system appeared to be an early trigger for Kupffer cell activation followed by induction of inflammatory cytokine interleukin-1beta synthesis leading to hepatic injury after partial hepatectomy PMID: 14572767
    11. CD14 glycoprotein is present on the cell membranes of vascular smooth muscle cells of rat aorta, where it acts as a lipopolysaccharide receptor. PMID: 15153652

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus.
  • 组织特异性：
    Detected in macrophages and peripheral blood monocytes.
  • 数据库链接：

    KEGG: rno:60350

    STRING: 10116.ENSRNOP00000023977

    UniGene: Rn.42942

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