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  4. Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

文献引用 (12) 说明书下载
  • 中文名称：
    大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶联免疫试剂盒
  • 货号：
    CSB-E08491r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶联免疫试剂盒（CSB-E08491r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TRACP-5b含量。TRACP-5b是一种靶点。它与骨代谢紧密相关，在破骨细胞中高度表达。研究机制方面，主要聚焦于其在破骨细胞活化、骨吸收过程中的作用。检测其水平变化，有助于评估骨代谢状态和相关疾病如骨质疏松等的发病机制。试剂盒检测范围为0.312 mIU/mL-20 mIU/mL，在骨质疏松机制研究、骨骼疾病模型评估及骨代谢相关药物开发等领域具有重要科研价值；适用于体外定量分析骨代谢相关动物模型的生物标志物表达水平，为骨生物学研究、药物干预效果评价及病理机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    TRACP-5b
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 mIU/mL-20 mIU/mL
  • 灵敏度：
    0.078 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %89-95
    1:2Average %100
    Range %97-103
    1:4Average %93
    Range %88-97
    1:8Average %98
    Range %93-102
  • 回收率：
    The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-96
    EDTA plasma (n=4)9591-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    202.076 1.988 2.032 1.887
    101.292 1.196 1.244 1.099
    50.804 0.820 0.812 0.667
    2.50.511 0.521 0.516 0.371
    1.250.324 0.335 0.330 0.185
    0.6250.282 0.278 0.280 0.135
    0.3120.223 0.212 0.218 0.073
    00.146 0.144 0.145 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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