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  4. Rat urokinase plasminogen activator,uPA ELISA kit

Rat urokinase plasminogen activator,uPA ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    大鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫试剂盒
  • 货号：
    CSB-E07368r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫试剂盒（CSB-E07368r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLAU含量。PLAU是一种重要的靶点。它参与多种生理病理过程，与肿瘤细胞的侵袭、转移密切相关。在研究机制方面，主要聚焦于其酶活性及信号通路，明确它对肿瘤微环境的调控作用，为开发针对性的抗肿瘤药物指明方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于心血管疾病、纤维化疾病或肿瘤相关动物模型中纤溶系统活性的动态监测，以及细胞迁移、组织修复等基础研究中的uPA表达水平分析，为科研人员探究uPA在病理生理过程中的调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    PlauUrokinase-type plasminogen activator ELISA kit; U-plasminogen activator ELISA kit; uPA ELISA kit; EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B] ELISA kit
  • 缩写：
    PLAU
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 88-95    
    1:2 Average % 95    
    Range % 90-100    
    1:4 Average % 97    
    Range % 94-103    
    1:8 Average % 101    
    Range % 96-105    
  • 回收率：
    The recovery of rat uPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 87-94    
    EDTA plasma (n=4) 96 94-101    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.334 2.186 2.260 2.077    
    500 1.652 1.625 1.639 1.456    
    250 1.047 0.997 1.022 0.839    
    125 0.636 0.688 0.662 0.479    
    62.5 0.398 0.402 0.400 0.217    
    31.2 0.325 0.337 0.331 0.148    
    15.6 0.246 0.232 0.239 0.056    
    0 0.189 0.177 0.183 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Plau Proteins

靶点详情

  • 功能：
    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：

    STRING: 10116.ENSRNOP00000014273

    UniGene: Rn.6064

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