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  4. Sheep Interleukin 1β,IL-1β ELISA Kit

Sheep Interleukin 1β,IL-1β ELISA Kit

文献引用 (10) 说明书下载
  • 中文名称：
    绵羊白介素1β(IL-1β)酶联免疫试剂盒
  • 货号：
    CSB-E10115Sh
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO绵羊白介素1β（IL-1β）酶联免疫检测试剂盒（货号：CSB-E10115Sh），采用双抗体夹心法原理实现精准定量分析，适用于检测血清、血浆及组织匀浆样本中IL-1β的蛋白表达水平。IL-1β作为关键的促炎性细胞因子，在机体免疫应答、炎症反应调控及感染性疾病机制研究中具有重要价值，其动态变化常被用于评估炎症相关疾病的病理进程。该试剂盒检测灵敏度达15.6 pg/mL，线性检测范围为15.6-1000 pg/mL，可稳定识别天然与重组蛋白，实验操作流程包含标准品梯度稀释、样本孵育、酶标抗体结合及显色反应等步骤，可在4.5小时内完成检测。科研人员可借助该试剂盒定量分析感染模型、自身免疫疾病研究或药物干预实验中不同生物样本的IL-1β浓度变化，为炎症机制探索、免疫调控研究及生物制剂开发提供可靠数据支持，所有检测结果仅用于科学研究目的。
  • 别名：
    IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
  • 缩写：
    IL1B
  • Uniprot No.：
  • 种属：
    Ovis aries (Sheep)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %83-90
    1:2Average %98
    Range %92-102
    1:4Average %100
    Range %96-104
    1:8Average %94
    Range %90-96
  • 回收率：
    The recovery of sheep IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-97
    EDTA plasma (n=4)9794-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.487 2.456 2.472 2.371
    5002.031 2.000 2.016 1.915
    2501.614 1.568 1.591 1.490
    1251.105 1.023 1.064 0.963
    62.50.703 0.689 0.696 0.595
    31.20.421 0.402 0.412 0.311
    15.60.275 0.262 0.269 0.168
    00.103 0.099 0.101 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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IL1B antibodies

IL1B Proteins

靶点详情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 基因功能参考文献：
    1. Intravenous injection of SB203580 successfully inhibited synthesis of IL-1beta and reduced the production of IL-6 in the hypothalamus. PMID: 24995301
    2. Interleukin-1beta was increased only on the injured side of multifidus in disc disease. PMID: 24718080
    3. Inactivation of RhoA due to lack of prenylation leads to Rac1 activation and subsequent priming for IL-1beta secretion. PMID: 25107911
    4. RANTES-403 was significantly associated with the risk of developing diffuse-type gastric cancer in men and found a possible gene-gene interaction between RANTES and IL-1B polymorphisms in gastric cancer carcinogenesis. PMID: 24323564
    5. In vitro studies with IL-1-beta and TNF-alpha challenges, both proinflammatory cytokines resulted in elevated levels of CCL5 in conditioned media (p<0.01) PMID: 24468005
    6. These results showed that IL-1beta and IL-1 receptor types I and II were expressed in the ovine hypothalamus in response to lipopolysaccharide injection. PMID: 20345592
    7. These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
    8. plasma IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes PMID: 17221363

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  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    KEGG: oas:443539

    UniGene: Oar.434

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