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中文名称：绵羊白介素4(IL-4)酶联免疫试剂盒
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货号：CSB-E15963Sh
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO绵羊白介素4（IL-4）酶联免疫检测试剂盒（货号：CSB-E15963Sh），采用双抗体夹心法定量检测血清、血浆及组织匀浆中的IL-4水平。IL-4是Th2淋巴细胞分泌的关键细胞因子，在调节体液免疫、B细胞活化及过敏反应中发挥重要作用，该试剂盒为研究绵羊免疫调节机制提供了可靠工具。其检测范围为6.25-400 pg/mL，灵敏度可达常规样本中IL-4的痕量检测需求，标准曲线线性相关系数优异，适用于免疫学研究、炎症性疾病模型分析、疫苗免疫效果评估等科研领域。实验操作采用预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统显色，支持分光光度计在450 nm波长下读取吸光值。样本兼容性强，可对多种生物样本进行平行检测，为探究绵羊感染性疾病、寄生虫免疫应答及自身免疫机制提供精准的蛋白定量数据，本产品仅用于科学研究用途。
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别名：IL4 ELISA Kit; IL-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
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缩写：
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Uniprot No.：
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种属：Ovis aries (Sheep)
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样本类型：serum, plasma, tissue homogenates
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检测范围：6.25 pg/mL-400 pg/mL
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灵敏度：1.56 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 86-95 1:2 Average % 102 Range % 97-105 1:4 Average % 95 Range % 90-97 1:8 Average % 97 Range % 93-100 -
回收率：
The recovery of sheep IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-97 EDTA plasma (n=4) 96 90-99 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 400 2.084 1.958 2.021 1.869 200 1.300 1.276 1.288 1.136 100 0.828 0.817 0.823 0.671 50 0.518 0.529 0.524 0.372 25 0.333 0.343 0.338 0.186 12.5 0.281 0.290 0.286 0.134 6.25 0.231 0.224 0.228 0.076 0 0.154 0.150 0.152 -
数据处理：
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货期：3-5 working days
引用文献
- Sheep immune‐stimulated with Saccharomyces boulardii show reduced prolificacy of Haemonchus contortus NB Pinheiro,Parasite Immunology,2022
- Induction of Interleukin-8 and Interleukin-12 in Neonatal Ovine Lung Following Experimental Inoculation of Bovine Respiratory Syncytial Virus E. Redondo et al,Journal of Comparative Pathology,2014
- Wnt2 promotes non-small cell lung cancer progression by activating WNT/ß-catenin pathway Chongbiao Huang et al,Am J Cancer Res,2015
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靶点详情
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功能：Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
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基因功能参考文献：
- Local production of the prototypic immunomodulatory cytokine IL-4 in transplanted corneal endothelium induces eosinophilia, inflammation, and rejection. PMID: 16081789
- Early IL-4 gene expression in abomasum is associated with resistance to Haemonchus infection. PMID: 27059919
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亚细胞定位：Secreted.
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蛋白家族：IL-4/IL-13 family
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数据库链接：
KEGG: oas:443327
UniGene: Oar.377
