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  4. Enterobacteria phage M13 III Monoclonal Antibody

Enterobacteria phage M13 III Monoclonal Antibody

Rare Species
说明书
  • 中文名称：
    大肠杆菌噬菌体M13 III鼠单克隆抗体
  • 货号：
    CSB-MA303116A0m
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: 1-3 lanes: Recombinant Enterobacteria phage M13 Attachment protein at 5ng, 10ng, 20ng; 4 lane: Enterobacteria phage M13 Attachment
      All lanes: Enterobacteria phage M13 III antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 65, 55-70 KDa
      Observed band size: 65, 55-70 KDa
      Exposure time：3min
  • 其他：

产品详情

  • 产品描述：
    Enterobacteria phage M13 III Monoclonal Antibody（CUSABIO货号：CSB-MA303116A0m）是一款高特异性科研用抗体，靶向M13噬菌体的III型结构蛋白。M13噬菌体作为丝状噬菌体家族代表，其衣壳蛋白在基因工程领域具有重要价值，广泛用于噬菌体展示技术构建重组蛋白表达系统。该抗体采用杂交瘤技术制备，经严格验证可特异性识别Enterobacteria phage M13的III型蛋白表位，在ELISA实验中展现高亲和力结合特性，适用于Western Blot检测重组噬菌体蛋白表达水平。产品经过交叉反应性测试，与大肠杆菌宿主蛋白无交叉反应，保障实验特异性，适用于噬菌体文库筛选、重组蛋白表达验证等分子生物学研究场景。作为噬菌体展示技术的关键检测工具，本产品可高效支持抗体库构建、靶向肽筛选及病毒样颗粒（VLP）研究，为基因工程、表位定位和蛋白质相互作用研究提供可靠试剂。实验方案已优化适配常规检测体系，满足科研人员对M13噬菌体相关实验的精准检测需求，是分子克隆与蛋白工程研究的理想选择。
  • 产品名称：
    Mouse anti-Enterobacteria phage M13 III Monoclonal antibody
  • Uniprot No.：
    P69168
  • 基因名：
    III
  • 别名：
    III antibody; Attachment protein G3P antibody; Gene 3 protein antibody; G3P antibody; Minor coat protein antibody
  • 宿主：
    Mouse
  • 反应种属：
    Enterobacteria phage M13
  • 免疫原：
    Recombinant Enterobacteria phage M13 Attachment protein (19-424AA)
  • 免疫原种属：
    Enterobacteria phage M13
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    1B8E8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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III antibodies

III Proteins

靶点详情

  • 功能：
    Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.
  • 亚细胞定位：
    Virion. Host membrane; Single-pass type I membrane protein. Note=Prior to assembly, G3P is found associated with the bacterial host inner membrane. There are about five copies of this protein per mature phage that are located on the head side of the filamentous virion.
  • 蛋白家族：
    Inovirus G3P protein family
  • 数据库链接：

    KEGG: vg:927334

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