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  4. SLN Monoclonal Antibody

SLN Monoclonal Antibody

说明书
  • 中文名称：
    SLN鼠单克隆抗体
  • 货号：
    CSB-MA021780A0m
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-MA021780A0m diluted at 1:60 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Overlay Peak curve showing U87 cells stained with CSB-MA021780A0m (red line) at 1:60. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    CUSABIO货号：CSB-MA021780A0m SLN单克隆抗体是针对肌集钙蛋白（Sarcolipin）靶点开发的高特异性科研试剂，该蛋白作为调节肌浆网钙ATP酶活性的关键因子，在骨骼肌和心肌细胞的钙离子稳态调控中发挥重要作用。本产品采用杂交瘤技术制备，经ELISA验证具有高亲和力，可兼容免疫组化（IHC）进行组织定位分析及流式细胞术（FC）检测细胞表面表达，适用于人类样本的蛋白质功能研究。其明确的物种特异性设计有效避免了交叉反应风险，为研究肌肉代谢机制、能量平衡调控及相关疾病模型构建提供可靠工具。在运动生理学、代谢性疾病基础研究及心肌功能探索等科研领域，该抗体可广泛应用于蛋白互作分析、亚细胞定位研究和分子信号通路解析，帮助科研人员深入探究SLN在肌细胞收缩舒张、产热调节中的分子机制。
  • 产品名称：
    Mouse anti-Homo sapiens (Human) SLN Monoclonal antibody
  • Uniprot No.：
    O00631
  • 基因名：
    SLN
  • 别名：
    SLN antibody; Sarcolipin antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human SLN protein (1-31AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    14G1A12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB WB：1:1000-1:5000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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SLN antibodies

SLN Proteins

靶点详情

  • 功能：
    Reversibly inhibits the activity of ATP2A1 and ATP2A2 in sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in muscle. Required for muscle-based, non-shivering thermogenesis.
  • 基因功能参考文献：
    1. Structure-Function Relationship of the SERCA Pump and Its Regulation by Phospholamban and Sarcolipin. PMID: 29594859
    2. Self-assembling study of sarcolipin and its mutants in multiple molecular dynamic simulations has been reported. PMID: 28241400
    3. These results suggest that sAnk1 interacts with SLN both directly and in complex with SERCA1 and reduces SLN's inhibitory effect on SERCA1 activity. PMID: 28487373
    4. analysis of transmembrane dynamics of the Thr-5 phosphorylated sarcolipin pentameric channel PMID: 27378083
    5. Phospholamban and sarcolipin are membrane proteins that differentially regulate SERCA function. (Review) PMID: 26743715
    6. Molecular dynamics simulations help to clarify and to understand better the SLN pentamer channel that had a hydrophobic gate and could switch Na(+) and Cl(-) ion permeability by hydrated and vacuum states. PMID: 26522287
    7. Although SLN and PLB binding to SERCA have different functional outcomes on the coupling efficiency of SERCA, both proteins decrease the apparent Ca(2+) affinity of the pump, suggesting that SLN and PLB inhibit SERCA by using a similar mechanism. PMID: 25983321
    8. SLN and PLN are co-expressed in most fibers, which suggests that super-inhibition of SERCAs may be physiologically important in the regulation of intracellular Ca2+ in human skeletal muscle. PMID: 24358354
    9. The C-terminal tail of SLN is a distinct, essential domain in the regulation of SERCA. PMID: 23362265
    10. The selective downregulation of SLN and enhanced sarcoplasmic reticulum Ca(2+) uptake in human AF suggest that SLN downregulation could play an important role in abnormal intracellular Ca(2+) cycling in atrial pathology. PMID: 21640081
    11. sarcolipin binds to phospholamban and inhibits polymerization PMID: 12032137
    12. sarcolipin is likely to be an atrial chamber-specific regulator of Ca2+ cycling in heart PMID: 12645548
    13. role in regulating sarco(endo)plasmic reticulum Ca2+-ATPase by binding to transmembrane helices alone or in association with phospholamban PMID: 12692302
    14. Gene expression of SLN was studied in children with congenital heart defects. PMID: 17515962

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  • 亚细胞定位：
    Sarcoplasmic reticulum membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Sarcolipin family
  • 数据库链接：

    HGNC: 11089

    OMIM: 602203

    KEGG: hsa:6588

    STRING: 9606.ENSP00000304707

    UniGene: Hs.334629

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