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中文名称：
lacZ鼠单克隆抗体
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货号：
CSB-MA009476A0m
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规格：
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图片：
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Western Blot
Positive WB detected in Recombinant protein
All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 117 kDa
Observed band size: 130 kDa
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Western Blot
Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
All lanes: lacZ antibody at 1:2000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 117 kDa
Observed band size: 130 kDa
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Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
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Immunoprecipitating lacZ in 293T transfected whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysate
Lane 2: CSB-MA009476A0m (2µl) + 293T transfected whole cell lysate (500µg)
Lane 3: 293T transfected whole cell lysate (20µg)
For western blotting, the blot was detected with CSB-MA000021M0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
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Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.
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其他：
产品详情
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产品描述：
CUSABIO货号：CSB-MA009476A0m单克隆抗体是专为大肠杆菌lacZ基因编码的β-半乳糖苷酶检测开发的科研工具，该酶在乳糖代谢中负责水解乳糖为可代谢单糖，同时作为分子生物学中广泛应用的报告基因标记。本产品采用杂交瘤技术制备，可特异性识别β-半乳糖苷酶抗原表位，适用于ELISA、Western Blot、免疫荧光、免疫沉淀及流式细胞术等多平台检测。在基因工程领域，该抗体可用于重组质粒筛选验证，通过特异性识别β-半乳糖苷酶表达产物辅助蓝白斑筛选系统；在蛋白相互作用研究中，可配合免疫共沉淀技术解析酶复合体形成机制；其流式检测功能为细菌代谢状态分析提供新维度。该抗体还可应用于基因表达调控研究，通过Western Blot定量分析lacZ报告基因在不同条件下的表达水平，为启动子活性检测和基因治疗载体构建提供关键技术支持。
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产品名称：
Mouse anti-Escherichia coli lacZ Monoclonal antibody
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Uniprot No.：
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基因名：
lacZ
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别名：
lacZ antibody; b0344 antibody; JW0335Beta-galactosidase antibody; Beta-gal antibody; EC 3.2.1.23 antibody; Lactase antibody
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宿主：
Mouse
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反应种属：
Escherichia coli
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免疫原：
Recombinant Escherichia coli Beta-galactosidase protein (2-1024AA)
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免疫原种属：
Escherichia coli
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标记方式：
Non-conjugated
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克隆类型：
Monoclonal
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抗体亚型：
IgG1
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纯化方式：
>95%, Protein G purified
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克隆号：
3B7D5
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, IF, IP, FC
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推荐稀释比：
| Application |
Recommended Dilution |
| WB |
1:1000-1:64000 |
| IF |
1:50-1:200 |
| IP |
1ul-4ul |
| FC |
1:100-1:300 |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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