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Recombinant Human G-protein coupled receptor 20 (GPR20)-VLPs (Active)

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  • 中文名称:
    人GPR20 Human-VLPs重组蛋白
  • 货号:
    CSB-MP860774HU
  • 规格:
    ¥5250
  • 图片:
    • CSB-MP860774HU is detected by Mouse anti-6*His monoclonal antibody.(This tag can be tested only under denaturing conditions.)
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Human GPR20 at 10 μg/ml can bind Anti-GPR20 recombinant antibody(CSB-RA860774MA1HU). The EC50 is 2.431 - 3.239 ng/mL.The VLPs (CSB-MP3838) is negative control. Biological Activity Assay
    • The purity of VLPs was greater than 95% as determined by SEC-HPLC
  • 其他:

产品详情

  • 纯度:
    Greater than 95% as determined by SEC-HPLC.
  • 生物活性:
    Measured by its binding ability in a functional ELISA. Immobilized Human GPR20 at 10 μg/mL can bind Anti-GPR20 recombinant antibody(CSB-RA860774MA1HU). The EC50 is 2.431 - 3.239 ng/mL.The VLPs (CSB-MP3838) is negative control.
  • 基因名:
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 蛋白长度:
    Full Length
  • 来源:
    Mammalian cell
  • 分子量:
    40.0 kDa
  • 表达区域:
    1-358aa
  • 氨基酸序列
    MPSVSPAGPSAGAVPNATAVTTVRTNASGLEVPLFHLFARLDEELHGTFPGLWLALMAVHGAIFLAGLVLNGLALYVFCCRTRAKTPSVIYTINLVVTDLLVGLSLPTRFAVYYGARGCLRCAFPHVLGYFLNMHCSILFLTCICVDRYLAIVRPEGSRRCRQPACARAVCAFVWLAAGAVTLSVLGVTGSRPCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLLHQGRQRRVRAMQLLLTVLIIFLVCFTPFHARQVAVALWPDMPHHTSLVVYHVAVTLSSLNSCMDPIVYCFVTSGFQATVRGLFGQHGEREPSSGDVVSMHRSSKGSGRHHILSAGPHALTQALANGPEA
  • 蛋白标签:
    C-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions)
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    3-7 business days
  • 注意事项:
    The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
  • Datasheet & COA:
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    Orphan receptor with constitutive G(i) signaling activity that activate cyclic AMP.
  • 基因功能参考文献:
    1. GPR20 constitutively activates G(i) proteins without ligand stimulation PMID: 18347022
  • 亚细胞定位:
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族:
    G-protein coupled receptor 1 family
  • 组织特异性:
    Ubiquitous with highest levels in intestinal tissues. In the brain detected in thalamus, putamen, and caudate, but not in frontal cortex, pons and hypothalamus.
  • 数据库链接:

    HGNC: 4475

    OMIM: 601908

    KEGG: hsa:2843

    STRING: 9606.ENSP00000366970

    UniGene: Hs.152432