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纯度:
Greater than 95% as determined by SDS-PAGE.
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内毒素:
Less than 1.0 EU/ug as determined by LAL method.
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生物活性:
Measured by its binding ability in a functional ELISA. Immobilized ICOSLG at 2 μg/ml can bind human ICOS (CSB-MP707478HU), the EC50 of human ICOSLG protein is 29.04-43.59 ng/ml.
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基因名:
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Uniprot No.:
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别名:
ICOSLG; B7H2; B7RP1; ICOSL; KIAA0653; ICOS ligand; B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD antigen CD275
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分子结构:
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种属:
Homo sapiens (Human)
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蛋白长度:
Partial
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来源:
Mammalian cell
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分子量:
28.9 kDa
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表达区域:
19-258aa
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氨基酸序列
DTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATWS
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蛋白标签:
C-terminal 6xHis-tagged
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产品提供形式:
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
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缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
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复溶:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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储存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
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保质期:
The shelf life is related to many factors, storage state,
buffer ingredients, storage temperature and the stability of the protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
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货期:
3-7 business days
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注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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产品描述:
Human ICOS ligand (ICOSLG) amino acid Asp19-Ser258, with an N-terminal linker, and a C-terminal 6xHis-tag, was expressed in mammalian cells. The product is the recombinant human ICOSLG protein. It is an active protein whose bioactivity has been measured by its binding ability with the human ICOS receptor in a functional ELISA. The EC50 of human ICOSLG protein is 29.04-43.59 ng/ml. The purity of this ICOSLG protein reaches up to 95% determined by SDS-PAGE. It has an apparent molecular mass of 40 kDa on SDS-PAGE while its predicted mass is 28.9 kDa. Glycosylation causes this result. Its endotoxi...
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Datasheet & COA:
Please contact us to get it.