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  4. Recombinant Human T-cell antigen CD7 (CD7), partial (Active)

Recombinant Human T-cell antigen CD7 (CD7), partial (Active)

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评价 (1)
  • 货号：
    CSB-MP004953HU
  • 规格：
    ￥828
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized CD7 at 5 μg/ml can bind SECTM1 (CSB-MP819898HU), the EC50 is 1.236-1.773 ng/ml. Biological Activity Assay
    • Activity
      Human CD7 protein hFc and Myc tag (CSB-MP004953HU) captured on COOH chip can bind Human SECTM1 protein hFc tag (CSB-MP819898HU) with an affinity constant of 1.84 nM as detected by LSPR Assay. Biological Activity Assay
    • The purity of CD7 was greater than 95% as determined by SEC-HPLC
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
    Greater than 95% as determined by SEC-HPLC.
  • 内毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized CD7 at 5 μg/ml can bind SECTM1 (CSB-MP819898HU), the EC50 is 1.236-1.773 ng/ml.②Human CD7 protein hFc and Myc tag (CSB-MP004953HU) captured on COOH chip can bind Human SECTM1 protein hFc tag (CSB-MP819898HU) with an affinity constant of 1.84 nM as detected by LSPR Assay.
  • 基因名：
    CD7
  • Uniprot No.：
    P09564
  • 别名：
    (GP40)(T-cell leukemia antigen)(T-cell surface antigen Leu-9)(TP41)
  • 分子结构：
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    Mammalian cell
  • 分子量：
    46.5 kDa
  • 表达区域：
    26-180aa
  • 氨基酸序列
    AQEVQQSPHCTTVPVGASVNITCSTSGGLRGIYLRQLGPQPQDIIYYEDGVVPTTDRRFRGRIDFSGSQDNLTITMHRLQLSDTGTYTCQAITEVNVYGSGTLVLVTEEQSQGWHRCSDAPPRASALPAPPTGSALPDPQTASALPDPPAASALP
  • 蛋白标签：
    C-terminal hFc-Myc-tagged
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    CUSABIO's product CSB-MP004953HU is a recombinant human T-cell antigen CD7. It is produced by the expression of the DNA fragment corresponding to 26-180 AA of the human CD7 protein in mammalian cells. This CD7 protein is fused with a hFc-Myc-tag at the C-terminus. It has been validated to be active through the functional ELISA (bind to the SECTM1, EC50 is 1.236-1.773 ng/ml) and LSPR assay (bind to human SECTM1 protein with an affinity constant of 1.84 nM). The purity of this CD7 protein is greater than 94.5% measured by SDS-PAGE. It contains less endotoxin, <1.0 EU/ug determined by the LAL ...显示更多
  • Datasheet & COA：
    Please contact us to get it.

产品评价

平均分:
5.0分 - 1 个评价

实验应用：结合实验/蛋白间互作

产品评价： 我使用CSB-MP004953HU产品进行ELISA实验，CD7在 5 μg/ml 可以结合人SECTM1，EC50为1.811-3.372ng/ml，蛋白有较好的结合活性，可以投入我后续研究应用。蛋白重复性好，产品采购方便，价格优惠，后续打算大量采购！

By 许老师

相关产品

CD7 antibodies

CD7 Proteins

靶点详情

  • 功能：
    Not yet known.
  • 基因功能参考文献：
    1. in acute myeloid leukemia patients the association of CD7 positivity and FLT3 positivity was found to be significant PMID: 25679063
    2. Data show that CD7 promotes extramedullary involvement of the B-ALL line Tanoue in an integrin beta2-dependent manner. PMID: 24920488
    3. We showed that the CADM1 versus CD7 plot is capable of discriminating clonally expanding HTLV-I-infected cells in indolent and acute-type T-cell leukemia-lymphomas and in asymptomatic carriers PMID: 24727323
    4. CD7 is present on monocytes and tumor macrophages and its ligand, SECTM1, is frequently expressed in corresponding melanoma tissues PMID: 24157461
    5. SECTM1 secreted from bone marrow stromal cells may interact with CD7 to influence GM-CSF expression in leukemic cells. PMID: 24211252
    6. epigenetic down-regulation of CD7 is associated with acute myeloid leukemia. PMID: 20398252
    7. CD7 loss in aggressive natural killer-cell leukemia; a useful diagnostic marker PMID: 20046078
    8. low expression in T-cell lymphomas due to Twist2-mediated suppression of promoter activity; enhanced by histone deacetylase inhibitors PMID: 19937140
    9. These findings indicate a link between epigenetic modifications and CD7 expression in primitive chronic myeloid leukemia cells. PMID: 20175919
    10. CD7 expression in hematopoitic cells denotes commitment to B-cell and natural killer cells lineages. PMID: 12393702
    11. Association of T cell antigen CD7 with type II phosphatidylinositol-4 kinase, a key component in pathways of inositol phosphate turnover. PMID: 12594831
    12. a novel fusion protein, designated scFvCD7:sFasL is designed to have leukemia-restricted activity. PMID: 16332967
    13. Patients expressing CD7 had significant shorter disease free (DFS) and post-remission survivals (PRS) than patients without CD7 (DFS of 12 months versus 42 months, P=0.005; PRS of 15 months versus 33 months, P=0.013). PMID: 16837044
    14. CD7 antigen notably expresses in lung microvascular endothelial cells (LME) and that it acts as an Fc receptor for IgM in LME cells. PMID: 16990185
    15. HTLV-IInfected cells acquired a profound decrease of intracellular calcium levels in response to ionomycin, timely correlated with decreased CD7 expression PMID: 17287851
    16. differential levels of CD7 identify the progressive stages of lineage commitment in human thymus, initiated from a primitive CD7(-) lympho-myeloid thymic progenitor PMID: 17959857
    17. close association of aberrant CD7 expression and FLT3/ITD mutation in the myeloblasts of FLT3/ITD+ acute myeloid leukemia suggests that FLT3/ITD- mediated leukemic transformation occurs in the more early stage of myeloid progenitor cells PMID: 18343790
    18. The CD7 expression in CD4(+) T cells discriminates between HL and reactive LAD, suggesting that this could be a useful and practical adjunctive tool in the diagnosis of Hodgkin lymphoma. PMID: 18956470
    19. by down-regulating CD7, ATLL cells could have escaped Gal-3-induced apoptosis to run a more aggressive clinical course PMID: 19207946
    20. the galectin-1 glycoprotein receptor CD7 maybe a novel target for GdA on T cells. PMID: 19683346

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 数据库链接：

    HGNC: 1695

    OMIM: 186820

    KEGG: hsa:924

    STRING: 9606.ENSP00000312027

    UniGene: Hs.36972

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