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AKT1 Antibody

  • 中文名称:
    AKT1兔多克隆抗体
  • 货号:
    CSB-PA149203
  • 规格:
    ¥2024
  • 图片:
    • Western blot analysis of extracts from 293 cells and HepG2 cells, using Akt antibody.
  • 其他:

产品详情

  • 产品名称:
    Rabbit anti-Homo sapiens (Human) AKT1 Polyclonal antibody
  • Uniprot No.:
  • 基因名:
  • 宿主:
    Rabbit
  • 反应种属:
    Human,Mouse,Rat
  • 免疫原:
    Synthesized peptide derived from N-terminal of Human Akt.
  • 免疫原种属:
    Homo sapiens (Human)
  • 克隆类型:
    Polyclonal
  • 纯化方式:
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA,WB
  • 推荐稀释比:
    Application Recommended Dilution
    WB 1:500-1:3000
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

靶点详情

  • 功能:
    AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to t...显示更多
  • 基因功能参考文献:
    1. The optimal melatonin concentration (3 mM) significantly decreased the intracellular reactive oxygen species levels, caspase-3 activity and the percentage of both dead and apoptotic-like sperm cells and increased the vitality, progressive motility and total motility and AKT phosphorylation compared with the control group. PMID: 29196809
    2. The findings indicate that SPRY4 and SPRY4-IT1 may act as oncogenes in testicular germ cell tumors via activation of the PI3K/Akt signaling pathway. PMID: 29410498
    3. Results suggest that transient receptor potential vanilloid 4 (TRPV4) accelerates glioma migration and invasion through the AKT/Rac1 signaling, and TRPV4 might be considered as a potential target for glioma therapy. PMID: 29928875
    4. Data indicate a regulatory mechanism underlying drug resistance and suggest that tribbles homologue 2 (TRIB2) functions as a regulatory component of the PI3K network, activating AKT in cancer cells. PMID: 28276427
    5. findings indicated that shikonin inhibits proliferation and promotes apoptosis in human endometrioid endometrial cancer (EEC) cells by modulating the miR-106b/PTEN/AKT/mTOR signaling pathway, suggesting shikonin could act a potential therapeutic agent in the EEC treatment. PMID: 29449346
    6. SIRT6 inhibited proliferation, migration, and invasion of colon cancer cells by up-regulating PTEN expression and down-regulating AKT1 expression. PMID: 29957460
    7. LHPP suppresses cell proliferation and metastasis in cervical cancer, and promotes apoptosis by suppressing AKT activation. PMID: 29944886
    8. Data show that activated proto-oncogene protein Akt (AKT) directly phosphorylates Fas associated factor 1 (FAF1) reduces FAF1 at the plasma membrane and results in an increase in TGF-beta type II receptor (TbetaRII) at the cell surface. PMID: 28443643
    9. Data show while overexpression of AKT serine/threonine kinase 1 (AKT1) promoted local tumor growth, downregulation of AKT1 or overexpression of AKT serine/threonine kinase 2 (AKT2) promoted peritumoral invasion and lung metastasis. PMID: 28287129
    10. High AKT1 expression is associated with metastasis in ovarian cancer. PMID: 29739299
    11. Circ-CFH promotes glioma progression by sponging miR-149 and regulating the AKT1 signaling pathway. PMID: 30111766
    12. High AKT1 expression is associated with metastasis via epithelialmesenchymal transition carcinoma in colorectal cancer. PMID: 30066935
    13. High AKT1 expression is associated with tumor-node-metastasis in nonsmall cell lung cancer. PMID: 30106450
    14. High expression of AKT1 is associated with drug resistance and proliferation of breast cancer. PMID: 28165066
    15. Germline variants in AKT1 gene are associated with prostate cancer. PMID: 29298992
    16. High AKT1 expression is associated with cisplatinresistant oral cancer. PMID: 29956797
    17. that Akt1 was a novel target for miR-637, and its knockdown also induced cell growth inhibition and apoptosis in pancreatic ductal adenocarcinoma cells PMID: 29366808
    18. High AKT1 expression is associated with periodontitis. PMID: 30218719
    19. High AKT1 expression is associated with angiogenesis of esophageal squamous cell carcinoma. PMID: 30015941
    20. High AKT1 expression is associated with Pancreatic Ductal Adenocarcinoma Metastasis. PMID: 29386088
    21. In MCF-7 cells, AIB1 overexpression increases p-AKT (Ser 473) activity.In both T47D and MCF-7 cells overexpressing A1B1, p-AKT (Ser 473) expression was significantly increased in the presence or absence of IGF-1,but increased more in presence of IGF-1. PMID: 29808803
    22. In this study, we used the Ion Personal Genome Machine (PGM) and Ion Torrent Ampliseq Cancer panel to sequence hotspot regions from PIK3CA, AKT and PTEN genes to identify genetic mutations in 39 samples of TNBC subtype from Moroccan patients and to correlate the results with clinical-pathologic data PMID: 30227836
    23. AKT pathway is activated by CBX8 in in hepatocellular carcinoma. PMID: 29066512
    24. Here the authors identified a direct interaction of both MEK1 and MEK2 with AKT. The interaction between MEK and AKT affects cell migration and adhesion, but not proliferation. The specific mechanism of action of the MEK-AKT complex involves phosphorylation of the migration-related transcription factor FoxO1. PMID: 28225038
    25. miR-195 suppresses cell proliferation of ovarian cancer cells through regulation of VEGFR2 and AKT signaling pathways. PMID: 29845300
    26. High AKT1 expression is associated with cell growth, aggressiveness, metastasis in gastric cancer. PMID: 30015981
    27. this is the first report showing long-duration exposure to nicotine causes increased proliferation of human kidney epithelial cells through activation of AKT pathway. PMID: 29396723
    28. RBAP48 overexpression contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3kinase/protein kinase B pathway suppression. PMID: 29901205
    29. Activating Akt1 mutations alter DNA double strand break repair and radiosensitivity. PMID: 28209968
    30. PI3K-Akt pathway inhibitors, Akti-1/2 and LY294002, reduced PFKFB3 gene induction by PHA, as well as Fru-2,6-P2 and lactate production. Moreover, both inhibitors blocked activation and proliferation in response to PHA, showing the importance of PI3K/Akt signaling pathway in the antigen response of T-lymphocytes. PMID: 29435871
    31. RIO kinase 3 (RIOK3) positively regulates the activity of the AKT/mTOR pathway in glioma cells. PMID: 29233656
    32. High AKT1 phosphorylation is associated with colorectal carcinoma. PMID: 29970694
    33. Results show that AKT1 was associated with hypertension in Mexican Mestizos but not Mexican Amerindians. PMID: 30176313
    34. TERT could induce thyroid carcinoma cell proliferation mainly through the PTEN/AKT signaling pathway. PMID: 29901196
    35. findings uncover a new function of p53 in the regulation of Akt signaling and reveal how p53, ASS1, and Akt are interrelated to each other. PMID: 28560349
    36. We performed quantitative mass spectrometry of IAV1918-infected cells to measure host protein dysregulation. Selected proteins were validated by immunoblotting and phosphorylation levels of members of the PI3K/AKT/mTOR pathway were assessed. PMID: 29866590
    37. Radiation resistance tumors have upregulated Onzin and POU5F1 expression. PMID: 29596836
    38. The essential role of AKT in the endocrine therapy resistance in estrogen receptor-positive, HER2-negative breast cancer.[review] PMID: 29086897
    39. FAL1 may work as a ceRNA to modulate AKT1 expression via competitively binding to miR-637 in HSCR. PMID: 30062828
    40. The overexpression of CHIP significantly increased the migration and invasion of the DU145 cells, which is possible due to activation of the AKT signaling pathway and upregulation of vimentin. The expression level of CHIP was observed to be increased in human prostate cancer tissues compared with the adjacent normal tissue. PMID: 29693147
    41. Genistein (GE) inhibited the growth of human Cholangiocarcinoma (CCA) cell lines by reducing the activation of EGFR and AKT, and by attenuating the production of IL6. E2 and ER were also involved in the growth-inhibitory effect of GE in CCA cells. PMID: 29693152
    42. this study identifies ORP2 as a new regulatory nexus of Akt signaling, cellular energy metabolism, actin cytoskeletal function, cell migration, and proliferation. PMID: 29947926
    43. The role of USP18 in breast cancer provides a novel insight into the clinical application of the USP18/AKT/Skp2 pathway. PMID: 29749454
    44. Collectively, these results indicate that COX-1/PGE2/EP4 upregulates the beta-arr1 mediated Akt signaling pathway to provide mucosal protection in colitis. PMID: 28432343
    45. AKT kinase pathway is regulated by SPC24 in breast cancer. PMID: 30180968
    46. CREBRF promotes the proliferation of human gastric cancer cells via the AKT signaling pathway. PMID: 29729692
    47. these results indicate that miR124 transection inhibits the growth and aggressive of osteosarcoma, potentially via suppression of TGFbetamediated AKT/GSK3beta/snail family transcriptional repressor 1 (SNAIL1) signaling, suggesting miR124 may be a potential anticancer agent/target for osteosarcoma therapy. PMID: 29488603
    48. piperine reduced the expression of pAkt, MMP9 and pmTOR. Together, these data indicated that piperine may serve as a promising novel therapeutic agent to better overcome prostate cancer metastasis. PMID: 29488612
    49. S100A8 gene knockdown reduced cell proliferation in the HEC-1A cells compared with control cells, induced cell apoptosis, inhibited the phosphorylation of protein kinase B (Akt), and induced the expression of pro-apoptotic genes. PMID: 29595187
    50. intact keratin filaments are regulators for PKB/Akt and p44/42 activity, basal and in response to stretch. PMID: 29198699

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  • 相关疾病:
    Breast cancer (BC); Colorectal cancer (CRC); Proteus syndrome (PROTEUSS); Cowden syndrome 6 (CWS6)
  • 亚细胞定位:
    Cytoplasm. Nucleus. Cell membrane.
  • 蛋白家族:
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily
  • 组织特异性:
    Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers dur
  • 数据库链接:

    HGNC: 391

    OMIM: 114480

    KEGG: hsa:207

    STRING: 9606.ENSP00000270202

    UniGene: Hs.525622