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  4. DDX3X Antibody

DDX3X Antibody

说明书
  • 中文名称：
    DDX3X兔多克隆抗体
  • 货号：
    CSB-PA006621LA01HU
  • 规格：
    ￥440
  • 图片：
    • Western Blot
      Positive WB detected in:293 whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate
      All lanes:DDX3 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 74 kDa
      Observed band size: 74 kDa
    • Immunohistochemistry analysis of human adrenal gland tissue using CSB-PA006621LA01HU at dilution of 1:100
    • Immunoprecipitating DDX3X in Jurkat whole cell lysate
      Lane 1: Rabbit control IgG (1µg) instead of CSB-PA006621LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA006621LA01HU (8µg) + Jurkat whole cell lysate (500µg)
      Lane 3: Jurkat whole cell lysate (10µg)
    • Immunofluorescence staining of HepG2 cell with CSB-PA006621LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with CSB-PA006621LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    Rabbits were immunized with recombinant human ATP-dependent RNA helicase DDX3X protein (2-662aa) to generate a polyclonal antibody that targets DDX3X. The resulting antibody was then purified to a level of greater than 95% purity using protein G affinity chromatography.

    This DDX3X polyclonal antibody can recognize and bind to DDX3X protein present in human, rat, and mouse samples. This DDX3X antibody has been thoroughly validated and is suitable for use in various applications, including ELISA, WB, IHC, IF, and IP. As a result, this antibody can be used to evaluate DDX3X protein level as well as to localize and isolate it.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) DDX3X Polyclonal antibody
  • Uniprot No.：
    O00571
  • 基因名：
    DDX3X
  • 别名：
    ATP dependent RNA helicase DDX3X antibody; ATP-dependent RNA helicase DDX3X antibody; CAP Rf antibody; DBX antibody; DDX14 antibody; DDX3X antibody; DDX3X_HUMAN antibody; DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody; DEAD (Asp-Glu-Ala-Asp) box helicase 3; X-linked antibody; DEAD box antibody; DEAD box protein 3 antibody; DEAD box protein 3 X-chromosomal antibody; DEAD box X isoform antibody; DEAD box; X isoform antibody; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody; DEAD/H box 3 antibody; DEAD/H box 3; X-linked antibody; Helicase like protein 2 antibody; Helicase-like protein 2 antibody; HLP2 antibody; X isoform antibody; X-chromosomal antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human ATP-dependent RNA helicase DDX3X protein (2-662AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，DDX3X Antibody (CSB-PA006621LA01HU)，的标记方式是Non-conjugated。对于DDX3X Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA006621LB01HU DDX3X Antibody, HRP conjugated ELISA
    FITC CSB-PA006621LC01HU DDX3X Antibody, FITC conjugated
    Biotin CSB-PA006621LD01HU DDX3X Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
    Immunoprecipitation (IP) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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DDX3X antibodies

DDX3X Proteins

靶点详情

  • 功能：
    Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs. Binds RNA G-quadruplex (rG4s) structures, including those located in the 5'-UTR of NRAS mRNA. Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities. Involved in transcription regulation. Positively regulates CDKN1A/WAF1/CIP1 transcription in an SP1-depend...显示更多
  • 基因功能参考文献：
    1. an N-terminal conserved Nuclear Export Signal (NES) is required for export of human DDX3 from the nucleus, and identified three regions within DDX3 that can independently facilitate its nuclear import. PMID: 30131165
    2. TRPV4 mediates Ca(2+) influx and nuclear accumulation of DDX3X in cells exposed to the Zika virus. Targeting of TRPV4 reduces infectivity of dengue, hepatitis C and Zika viruses. Our results highlight the role of TRPV4 in the regulation of DDX3X-dependent control of RNA metabolism and viral infectivity. PMID: 29899501
    3. DDX3 regulates MTP gene expression and lipid homeostasis through interplay with HNF4 and SHP. PMID: 28128295
    4. Through adopting the immunoprecipitation (IP), RNA immunoprecipitation (RIP), dual luciferase reporter assays, the authors illustrate that DDX3X could interact with Drosha/DGCR8 complex, elevate the processing activity of Drosha/DGCR8 complex on pri-miRNAs, and increase mature miRNA expression levels. PMID: 27586307
    5. our study suggested that DDX3 prevents generation of cancer stem cells through epigenetically regulating a subset of tumor-suppressive miRNAs expressions, which strengthens tumor suppressor role of DDX3 in hepatocellular carcinoma. PMID: 27344963
    6. this study shows that cancer-associated DDX3X mutations drive stress granule assembly and impair global translation PMID: 27180681
    7. we demonstrated that DDX3 modulated the activity of PP2A by controlling the phosphorylation of PP2A-C, which might enable PP2A-C to regulate NF-kappaB signal pathway by dephosphorylating IKK-beta. PMID: 28402257
    8. Data suggest that L protein from LCMV interactions with host proteome, specifically DDX3X, NKRF, and TRIM21. (LCMV = Lymphocytic choriomeningitis mammarenavirus; DDX3X = DEAD-box helicase 3; NKRF = NF-kappa-B-repressing factor; TRIM21 = tripartite motif-containing protein-21) PMID: 29261807
    9. DDX3 interacts extensively with RNA and ribosomal machinery to help remodel the translation landscape in response to stress, while cancer-related DDX3 variants adapt this response to selectively preserve translation PMID: 27058758
    10. Mechanistically, increased KRAS expression induced ROS production, which elevated HIF-1alpha and YAP1 expression. Increased HIF-1alpha persistently promoted DDX3 expression via a KRAS/ROS/HIF-1alpha feedback loop. PMID: 28435452
    11. Our study suggests that rottlerin exhibits its anti-cancer activity partly due to upregulation of DDX3 in hepatocellular carcinoma cells. PMID: 29203243
    12. DDX3 may play an oncogenic role to promote tumor growth and invasion in colon cancer cells PMID: 27007150
    13. Data suggest that DEAD-box helicase 3 (DDX3X) physically interacts and co-localizes with poly(A)-binding cytoplasmic protein 1 (PABPC1) and caprin-1 in lamellipodia at the leading edge of spreading cells; these interactions are dependent on mRNA; depletion of DDX3X (via gene silencing with the CRISPR-Cas system) leads to decreased cell motility. These studies were conducted using MRC5 lung fibroblast cell line. PMID: 28733330
    14. The article describes RNA remodeling activity of human DDX3X and Caenorhabditis elegans LAF-1 tuned by protein concentration, RNA length, and ATP. PMID: 27546789
    15. the role of DDX3 in sarcomas PMID: 26364611
    16. DDX3 directly regulates TRAF3 ubiquitination and acts as a scaffold to co-ordinate assembly of signaling complexes downstream from MAVS. PMID: 27980081
    17. Here we identify the DEAD-box helicase 3 (DDX3) as a novel interaction partner of Y. enterocolitica YopM and present the three-dimensional structure of a YopM:DDX3 complex PMID: 27300509
    18. Here, the authors show that herpes simplex virus 1 gene expression, replication, and propagation depend on optimal DDX3X protein levels. PMID: 28148788
    19. de novo heterozygous DDX3X variants should be considered not only in females with unexplained ID, but also in individuals with a clinical diagnosis of T-CS. PMID: 28371085
    20. high metastatic DDX3 expression correlates with worse survival, implying that DDX3 is a potential therapeutic target in metastatic breast cancer, in particular in the clinically important group of TN patients. PMID: 27999982
    21. Herein, we showed for the first time, to our knowledge, that the inhibition of DDX3 by a small molecule could be successfully exploited for the development of a broad spectrum antiviral agent. PMID: 27118832
    22. Data show that knockdown of RNA helicase DDX3 in breast cancer MCF-7 and MDA-MB-231 cells resulted in decreased proliferation rates. PMID: 26337079
    23. Our results suggest that the intrinsically disordered N-terminal domain of DDX3 regulates its functions in translation by acting prior to the recruitment of the 43S pre-initiation complex onto the viral 5'-UTR. PMID: 27012366
    24. The results do not support our hypothesis that common germline genetic variants in the DDX3X genes is associated with the risk of developing medulloblastoma. PMID: 26290144
    25. analysis of the structural and functional core of the DDX3 subfamily of DEAD-box proteins PMID: 26598523
    26. The DDX3 may participate in antiviral innate immunity, at least in part, by translational control of interferon-induced protein kinase (PACT). PMID: 26454002
    27. As such, DDX3 has been shown to play roles both upstream and downstream of I-kappa beta kinase epsilon (IKKepsilon)/TANK-binding kinase 1, leading to IFN-beta production. PMID: 26174373
    28. Data show that DEAD-box helicase 3 (DDX3) had a significant prognostic predictive power in colorectal cancer at both RNA and protein level. PMID: 26087195
    29. Taken together, our result demonstrates that Ketorolac salt is a newly discovered bioactive compound against DDX3 and this compound can be used as an ideal drug candidate to treat DDX3 associated oral cancer. PMID: 25918862
    30. Loss of DDX3 function either by shRNA or by RK-33 impaired Wnt signaling through disruption of the DDX3-beta-catenin axis and inhibited non-homologous end joining-the major DNA repair pathway in mammalian somatic cells. PMID: 25820276
    31. T-cell lymphoma patients with DDX3X mutations presented a poor prognosis. PMID: 26192917
    32. Either ligand-independent or ligand-induced EGFR phosphorylation was inhibited in lung cancer cells that strongly expressed DDX3X. PMID: 25343452
    33. Mutations in DDX3X are a common cause of unexplained intellectual disability with gender-specific effects on Wnt signaling. PMID: 26235985
    34. Data suggest complex translational control mechanism(s) for the human DDX3X gene locus functioning only in the male germ line and resulting in expression of its protein only in the postmeiotic spermatids. PMID: 25208899
    35. Low/negative DDX3 expression in tumor cells was significantly associated with aggressive clinical manifestations and might be an independent survival predictor, particularly in non-smoker patients with OSCC PMID: 23410059
    36. identification of DDX3X mutations in 10% of cases, preferentially in males (4/5 cases); analysis suggested an association between DDX3X inactivation and clinically unfavorable features and poor outcome of chronic lymphocytic leukaemia PMID: 25382417
    37. The DDX3-Rac1-beta-catenin regulatory axis in modulating the expression of Wnt/beta-catenin target genes. PMID: 25043297
    38. Upon infection, the HCV 3'UTR redistributes DDX3X and IKK-alpha to speckle-like cytoplasmic structures shown to be stress granules. PMID: 25740981
    39. Cancer-associated mutants of RNA helicase DDX3X are defective in RNA-stimulated ATP hydrolysis. PMID: 25724843
    40. Mutations in DDX3X gene is associated with recurrent convergent evolution in chronic lymphocytic leukemia. PMID: 25377784
    41. DDX3X, a member of DEAD-box RNA helicase, is necessary for IFN production and could inhibit DENV replication PMID: 25437271
    42. This review discusses the considerable body of work on the biochemistry and biology of DDX3, including the recently discovered link of human DDX3 to tumorigenesis. PMID: 25039764
    43. Overall, these results demonstrate that DDX3 represents an intrinsic host antiviral factor that restricts hepatitis B virus transcription. PMID: 25231298
    44. These results suggest that anti-DDX3X immunotherapy is a promising treatment option in efforts to eradicate CSC in the clinical setting. PMID: 23974721
    45. DDX3 loss by p53 inactivation via MDM2/Slug/E-cadherin pathway promotes tumor malignancy and poor patient outcome PMID: 23584477
    46. Host DDX3 regulates Japanese encephalitis virus replication by interacting with viral un-translated regions. PMID: 24418539
    47. DDX3 seems to interact with the HIV-1 Tat and facilitate the Tat function. PMID: 24183723
    48. DDX3 is a new key molecule to understand the molecular mechanism underlying RNAi pathway in mammals. PMID: 23527197
    49. Results suggest that distinct DDX DEAD-box RNA helicases DDX3 and DDX5 cooperate to modulate the HIV-1 Rev function. PMID: 23608157
    50. In pediatric T-acute lymphoblastic leukemia, we have identified 2 RNA processing genes, that is, HNRNPH1/5q35 and DDX3X/Xp11.3 as new MLLT10 fusion partners. PMID: 23673860

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  • 相关疾病：
    Mental retardation, X-linked 102 (MRX102)
  • 亚细胞定位：
    Cell membrane. Nucleus. Cytoplasm. Cytoplasm, Stress granule. Inflammasome. Cell projection, lamellipodium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    DEAD box helicase family, DDX3/DED1 subfamily
  • 组织特异性：
    Widely expressed. In testis, expressed in spermatids. Expressed in epidermis and liver (at protein level).
  • 数据库链接：

    HGNC: 2745

    OMIM: 300160

    KEGG: hsa:1654

    STRING: 9606.ENSP00000382840

    UniGene: Hs.728563

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