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中文名称：
ZDHHC1兔多克隆抗体
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货号：
CSB-PA837848LA01HU
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规格：
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图片：
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Western Blot
Positive WB detected in: A549 whole cell lysate(20µg), Hela whole cell lysate(20µg), U251 whole cell lysate(20µg), 293 whole cell lysate(20µg), Mouse Brain whole cell lysate(20µg),Mouse Spleen tissue lysate(20µg)
All lanes: ZDHHC1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 55 kDa
Observed band size: 55 kDa
Exposure time: 10s
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IHC image of CSB-PA837848LA01HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Immunofluorescence staining of U251 cell with CSB-PA837848LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescence staining of A549 cell with CSB-PA837848LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
产品详情
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产品名称：
Rabbit anti-Homo sapiens (Human) ZDHHC1 Polyclonal antibody
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Uniprot No.：
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基因名：
ZDHHC1
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别名：
ZDHHC1; C16orf1; ZNF377; Palmitoyltransferase ZDHHC1; DHHC domain-containing cysteine-rich protein 1; Zinc finger DHHC domain-containing protein 1; DHHC-1; Zinc finger protein 377
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宿主：
Rabbit
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反应种属：
Human, Mouse
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免疫原：
Synthesized peptide derived from human ZDHC1 AA range: 81-131
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免疫原种属：
Homo sapiens (Human)
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克隆类型：
Polyclonal
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抗体亚型：
IgG
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纯化方式：
Antigen affinity purification
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, IHC, IF
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推荐稀释比：
| Application |
Recommended Dilution |
| WB |
1:1000-1:3000 |
| IHC |
1:50-1:200 |
| IF |
1:20-1:100 |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
靶点详情
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功能：
Palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates. Has a palmitoyltransferase activity toward NCDN and regulates NCDN association with endosome membranes through this palmitoylation.; Has also a palmitoyltransferase activity-independent function in DNA virus-triggered and CGAS-mediated innate immune response. Functions as an activator of STING1 by promoting its cGAMP-induced oligomerization and the recruitment of downstream signaling components.
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基因功能参考文献：
- In an expression screen for proteins that can activate the IFNB1 promoter, authors identified the ER-associated protein ZDHHC1 as a positive regulator of virus-triggered, MITA/STING-dependent immune signaling. PMID: 25299331
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亚细胞定位：
Endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus.
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蛋白家族：
DHHC palmitoyltransferase family
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组织特异性：
Widely expressed with significant expression in heart, brain, placenta, lung, liver, kidney, testis, thymus and small intestine. Expressed at lower levels in adult pancreas and lung.
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数据库链接：
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