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  4. CD147 Recombinant Monoclonal Antibody

CD147 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    CD147重组抗体
  • 货号：
    CSB-RA002831A0HU
  • 规格：
    ￥1320
  • 图片：
    • The Binding Activity of CD147 with Anti-CD147 recombinant Antibody
      Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD147 (CSB-MP002831HU1) at 2 μg/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21.95-33.12 ng/ml.
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, ntera2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate
      All lanes: CD147 antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42, 29, 23, 19 KDa
      Observed band size: 35, 50-60 KDa
    • IHC image of CSB-RA002831A0HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA002831A0HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA002831A0HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA002831A0HU diluted at 1:200 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cells with CSB-RA002831A0HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA002831A0HU (red line) with 1 μg/well (10 μg/mL, 100 μL/well). Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions.Acquisition of >10,000 events was performed.
    • Overlay Peak curve showing Jurkat cells stained with CSB-RA002831A0HU (red line) with 1 μg/well (10 μg/mL, 100 μL/well). Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions.Acquisition of >10,000 events was performed.
    • Overlay Peak curve showing HepG2 cells surface stained with CSB-RA002831A0HU (red line) with 1 μg/well (10 μg/mL, 100 μL/well). Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions.Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    CD147重组单克隆抗体（CUSABIO货号：CSB-RA002831A0HU）是一种高特异性抗体，靶向细胞膜表面糖蛋白CD147（又称Basigin或EMMPRIN），该分子在肿瘤发生、炎症反应及病原体感染中发挥关键作用，可通过调控基质金属蛋白酶（MMPs）分泌及细胞间相互作用参与多种病理生理过程。本抗体采用重组技术制备，经ELISA、蛋白质印迹（WB）、免疫组化（IHC）、免疫荧光（IF）和流式细胞术（FC）等多平台严格验证，结果显示其在不同实验体系中均表现出优异的灵敏度和特异性。推荐使用稀释范围为：WB 1:500-1:2000，IHC 1:50-1:200，IF 1:50-1:200，FC 1:50-1:200。该产品适用于体外研究CD147在细胞迁移、增殖调控、代谢重编程及肿瘤微环境重塑中的分子机制，尤其适用于探索其在癌症生物学、免疫细胞活化及病毒宿主相互作用等领域的潜在功能，为细胞信号转导研究和跨膜蛋白功能解析提供可靠工具。
  • Uniprot No.：
    P35613
  • 基因名：
    BSG
  • 别名：
    5A11 antigen antibody; 5F7 antibody; BASI_HUMAN antibody; Basigin (Ok blood group) antibody; Basigin antibody; Blood brain barrier HT7 antigen antibody; Bsg antibody; CD 147 antibody; CD147 antibody; CD147 antigen antibody; Collagenase stimulatory factor antibody; EMMPRIN antibody; Extracellular matrix metalloproteinase inducer antibody; Leukocyte activation antigen M6 antibody; M 6 antibody; M6 antibody; M6 leukocyte activation antigen antibody; Neurothelin antibody; OK antibody; OK blood group antibody; OK blood group antigen antibody; TCSF antibody; Tumor cell derived collagenase stimulatory factor antibody; Tumor cell-derived collagenase stimulatory factor antibody
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human CD147 protein
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    mIgG2a
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    11F3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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靶点详情

  • 功能：
    Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors. May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes.; Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chem...显示更多
  • 基因功能参考文献：
    1. ectopic CD147-polarized distribution on basolateral membrane promotes hepatocyte depolarization by activation of the CD147-integrin alpha5beta1-E-cadherin ubiquitination-partitioning defective 3 decrease and beta-catenin translocation signaling cascade, replenishing a molecular pathway in hepatic carcinogenesis PMID: 29356040
    2. CD147 and the beta2-adrenergic receptor form hetero-oligomeric complexes.Actinin-4 directly binds to the cytosolic tail of CD147 and governs the assembly of CD147-beta2-adrenergic receptor complexes in highly ordered clusters at bacterial adhesion sites. PMID: 28569760
    3. Plasma sCD147 levels are elevated in breast cancer patients. Soluble CD147 is also associated with tumour size, lymph node metastasis, high recurrent risk, and chemoresistance. PMID: 29807951
    4. overexpression of CD147 may promote OSF progression. PMID: 29457855
    5. these findings show that CD147 is a novel and key mediator of IL-22-induced psoriatic alterations in the epidermis and might be a therapeutic target in patients with psoriasis. PMID: 28272440
    6. Data indicate that CD147 promotes breast cancer cell proliferation, metastasis, and invasion by modulating matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor (VEGF) expression. PMID: 29901696
    7. BSG expression is indicative of a poor prognosis in pulmonary adenocarcinoma PMID: 29431238
    8. CD147 expression was associated with clinicopathological features and biomarkers related to triple-negative breast cancer, particularly basal-like breast cancer. PMID: 30099407
    9. Characterization of the role of CD147 in the development of tumors should lead to a better understanding of the changes occurring at the molecular level during the development and progression of primary human bone cancer. PMID: 30043854
    10. Results find CD147 expression level increased in colorectal cancer (CRC) tumors and cell lines and inversely correlated with mir-485 expression. Also, CD147 is a target gene of miR485 which negatively regulates its gene expression. PMID: 29532886
    11. Our results indicate that concomitant stimulation and colocalization of galectin-3 with CD147 are associated with increased gelatinolytic activity in the actively ulcerating human cornea PMID: 29340650
    12. These findings reveal an important cytomegalovirus mechanism for evading antiviral innate immunity through its encoded microRNA US25-1-5p by targeting transmembrane glycoprotein CD147. PMID: 29194430
    13. on a major part of fibroblastic cells in encapsulating peritoneal sclerosis PMID: 28551820
    14. Review: Bsg is an extracellular receptor for CyPA that promotes cell proliferation and inflammation. Thus, the CyPA/Bsg system is potentially a novel therapeutic target for cardiovascular diseases. PMID: 28993547
    15. protein-protein interaction studies on TRAF6 and BSG to get molecular level insights of the reactions. PMID: 28612997
    16. results showed that the rs4919862 SNP of CD147 was closely associated with carotid atherosclerotic plaques formation; thus, polymorphisms of the CD147 gene may be related to the tendency for carotid atherosclerotic plaques PMID: 28582638
    17. Results indicated that higher expression of CD147/EMMPRIN potentially may be a prognostic marker for most cancers. CD147/EMMPRIN overexpression predicted a high risk for chemotherapy drugs resistance. CD147/EMMPRIN is a central player in tumor progression and predicts a poor prognosis. [review] PMID: 27608940
    18. BSG mutation is associated with early-onset high myopia. PMID: 28373534
    19. Cancer cells and fibroblasts interaction promotes breast cancer cells showing stem-like cell properties through up-regulation of EMMPRIN. PMID: 27325313
    20. Cholesterol depletion inhibits the endocytosis of CD147 but that the constitutive shedding of CD147 mediated by ADAM10 is enhanced. PMID: 28703811
    21. Overexpression of CD147 support the metabolic reprograming in papillary renal cell carcinoma PMID: 28028797
    22. This research found that CD147 is correlated with aggressiveness of the glioma, tumor grades, tumor size, necrosis, overall survival, and recurrence rate. PMID: 28560663
    23. Upregulation of CD147 Promotes Metastasis of Cholangiocarcinoma by Modulating the Epithelial-to-Mesenchymal Transitional Process PMID: 28244853
    24. Our retrospective analysis demonstrated CD147 protein expression was significantly associated with clinical N stage, and tumor stage. Meanwhile, it can also serve as an independent prognosis biomarker. PMID: 28427166
    25. miR-492 may be involved in the regulation of OK antigen expression on red blood cells with the BSG rs8259 TT genotype. PMID: 28981932
    26. Our findings show that emmprin is released through microvesicle shedding in sarcoma cells, and emmprin in microvesicles regulates MMP-2 production by influencing the activity of fibroblasts located at sites distant from the tumor cells. PMID: 28498412
    27. enhancement of the effect of trastuzumab on HER2-positive cells following CD147 knockdown might be attributed to increased apoptosis and decreased phosphorylation of signaling proteins PMID: 27363028
    28. this study shows that CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma PMID: 27556188
    29. Fucosylation of CD147 by Lewis y antigen enhanced the survival ability by promoting basic autophagy activity and restraining autophagic cell death in ovarian cancer. PMID: 27863372
    30. Review/Meta-analysis: up-regulation of CD147 was effectively predictive of worse prognosis in gastrointestinal cancer. PMID: 27768590
    31. Suggest a central role for CD147 in pancreatic cancer metabolic reprogramming, particularly with respect to amino acid anabolism and calcium signaling. PMID: 28039486
    32. combined CA9-/CD147-capture antibodies demonstrated high efficiency for capturing circulating renal cell carcinoma cells PMID: 27494883
    33. important factor in the aggressive behaviour of melanoma PMID: 27060463
    34. Xkr8-BSG/NPTN complex is required for phosphatidylserine scrambling in apoptotic cell membranes. PMID: 27503893
    35. Our study provides new evidence that interaction of KLF6 and Sp1 regulates basigin-2 expression in hepatocellular carcinoma PMID: 27057625
    36. EMMPRIN/MMPs/VEGF pathway is involved in PDR angiogenesis. PMID: 27860331
    37. study identified novel interactions between CD147 and RING1, recovered CD147 nuclear envelope distribution in melanoma cells, and suggested a new mechanism underlying how cytoplasmic CD147 promotes melanoma development PMID: 28832687
    38. Immunohistochemistry was employed to analyze cFos, cJun and CD147 expression in 41 UCB cases and 34 noncancerous human bladder tissues. PMID: 28358415
    39. High BSG expression is associated with multiple myeloma. PMID: 28017969
    40. report that a phosphoserine (pSer) in CD147 (pSer252) is responsible for this interaction and inhibition of the Smad4/p21(WAF1) signal that promotes cell proliferation PMID: 28684116
    41. findings indicate that Rab22a enhances recycling of CD147, which is required for lung cancer cell migration and invasion,and targeting CD147 recycling may be a rational strategy for lung cancer therapy PMID: 28433697
    42. Studied expression of CD147 in both ALK+ and ALK- anaplastic large-cell lymphoma; found CD147 to be a downstream target for activation by C/EBPbeta. PMID: 28581487
    43. this studies identify potential signaling routes of CD147 affecting T cell growth and function PMID: 28148733
    44. Our results highlight the important role of the TGF-beta1-CD147 self-sustaining network in driving HCC development by regulating differentiation plasticity, which provides a strong basis for further investigations of the differentiation therapy of HCC targeting TGF-beta1 and CD147. PMID: 27041581
    45. rs8259 polymorphism failed to exhibit an association with cardiovascular mortality (p = 0.283). BSG rs8259 polymorphism may contribute to decreased risk of Chronic Heart Failure in a Chinese Han population PMID: 28230811
    46. This study suggests that CD44 and CD147 together improve the prognostic efficacy of tumor differentiation; in vitro results further point out that these markers might be determinant of differentiation characteristics, imparting properties of increased self-renewal, migration, and invasion. PMID: 28631562
    47. Results suggest that CD147 promotes chemoresistance by activating MAPK/ERK signaling pathway in head and neck squamous cell carcinoma (HNSCC). PMID: 28062212
    48. PvTRAg38 binds to two erythrocyte receptors basigin and band 3 through P2 and P4 regions, respectively, to facilitate parasite growth. PMID: 27881677
    49. phosphatidylinositol 3-kinase/Akt pathway may be the probable signaling pathway underlying CD147 induced up-regulation of VEGF in U937-derived foam cells PMID: 27619643
    50. D147 expression is potentially closely related to Hepatocellular Carcinoma survival and associated clinicopathological parameters, paving the way for further research PMID: 28386553

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  • 亚细胞定位：
    Melanosome.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein. Endosome. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein.; [Isoform 4]: Cell membrane; Single-pass type I membrane protein.
  • 组织特异性：
    [Isoform 1]: Retina-specific. Expressed in retinal cone photoreceptors (at protein level).; [Isoform 2]: Expressed in erythrocytes (at protein level). Highly expressed in melanoma cell lines (at protein level). Highly expressed in the heart, kidney, skele
  • 数据库链接：

    HGNC: 1116

    OMIM: 109480

    KEGG: hsa:682

    STRING: 9606.ENSP00000333769

    UniGene: Hs.501293

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