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  4. LGR6 Recombinant Monoclonal Antibody

LGR6 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    LGR6重组抗体
  • 货号：
    CSB-RA156336A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA156336A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.26% DAB.
    • Immunofluorescence staining of hela with CSB-RA156336A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 500-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells surface stained with CSB-RA156336A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA156336A0HU LGR6重组单克隆抗体是经过严格验证的高特异性科研工具，靶向LGR6（Leucine-rich repeat-containing G-protein coupled receptor 6），该蛋白属于G蛋白偶联受体家族成员，在干细胞调控及组织再生中发挥重要作用，尤其与表皮干细胞分化、毛囊发育及肿瘤发生相关。本抗体通过ELISA实验验证可特异性识别天然及重组LGR6蛋白，免疫组化（IHC）实验显示其在石蜡包埋组织中能清晰定位细胞膜表达，免疫荧光（IF）染色呈现高分辨率的亚细胞定位信号，流式细胞术（FC）进一步证实其适用于活细胞表面抗原检测。推荐工作浓度为IHC/IF/FC 1:50-1:200，可满足多样本类型的实验需求。该产品适用于干细胞生物学、组织再生机制研究、肿瘤微环境分析及发育生物学等领域，尤其为探索LGR6在表皮稳态维持、癌症干细胞标志物筛选等方向提供可靠检测工具。本产品仅供科学研究使用，需根据实验体系优化具体条件。
  • Uniprot No.：
    Q9HBX8
  • 基因名：
    LGR6
  • 别名：
    Leucine-rich repeat-containing G-protein coupled receptor 6, LGR6
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human LGR6
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    15G3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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LGR6 antibodies

LGR6 Proteins

靶点详情

  • 功能：
    Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. May act as a tumor suppressor.
  • 基因功能参考文献：
    1. Inhibition of LGR6 promoted the osteogenic differentiation of bone marrow stromal cells in vitro. PMID: 29625528
    2. Lgr6 has a tumorpromoting role in the development of colorectal cancer. PMID: 29693156
    3. Lgr6 marks mammary gland progenitor cells that can initiate tumours, and cells of luminal breast tumours required for efficient tumour maintenance. PMID: 27798604
    4. Two of the three monoclonal antibodies recognized the large N-terminal extracellular domain of leucine rich repeat containing G protein-coupled receptor 6 (LGR6), and competitively blocked the binding of R-spondin 1, which is known to be the ligand for LGR6. PMID: 28013222
    5. LGR6 expression is significantly downregulated in human masticatory mucosa during wound healing PMID: 28005267
    6. Overexpression of wild-type LGR6 in HeLa cells leads to increased cell migration. PMID: 22615920

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 数据库链接：

    HGNC: 19719

    OMIM: 606653

    KEGG: hsa:59352

    STRING: 9606.ENSP00000356247

    UniGene: Hs.497402

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