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  4. MAPK10 Recombinant Monoclonal Antibody

MAPK10 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    MAPK10重组抗体
  • 货号：
    CSB-RA240216A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: PC-3 whole cell lysate, Mouse brain tissue
      All lanes: MAPK10 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 53, 49, 32kDa
      Observed band size: 55 kDa
    • IHC image of CSB-RA240216A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of PC-3 cell with CSB-RA240216A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 553-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing PC3 cells stained with CSB-RA240216A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    MAPK10重组单克隆抗体（产品编号CSB-RA240216A0HU）是针对丝裂原活化蛋白激酶10（MAPK10/JNK3）开发的高特异性抗体，适用于多种实验场景。该抗体靶向的MAPK10是c-Jun氨基末端激酶（JNK）家族成员，在调控细胞应激反应、程序性死亡及神经元分化中起关键作用，尤其在神经系统中高度表达，与阿尔茨海默病、帕金森病等病理机制相关。经严格验证，本抗体在Western Blot中可检测到单一清晰目标条带，免疫组化与免疫荧光实验显示明确的亚细胞定位信号，流式细胞术验证其与天然蛋白的高亲和力。推荐稀释比例为：WB 1:500-1:2000、IHC 1:50-1:200、IF 1:50-1:200、FC 1:50-1:200，不同批次间性能稳定。该产品适用于探究神经退行性疾病分子机制、细胞应激信号通路研究以及药物靶点筛选等科研领域，为体外实验提供可靠工具。
  • Uniprot No.：
    P53779
  • 基因名：
    MAPK10
  • 别名：
    Mitogen-activated protein kinase 10 (MAP kinase 10) (MAPK 10) (EC 2.7.11.24) (MAP kinase p49 3F12) (Stress-activated protein kinase 1b) (SAPK1b) (Stress-activated protein kinase JNK3) (c-Jun N-terminal kinase 3), MAPK10, JNK3 JNK3A PRKM10 SAPK1B
  • 反应种属：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human MAPK10
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    29E10
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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MAPK10 antibodies

MAPK10 Proteins

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transc...显示更多
  • 基因功能参考文献：
    1. Mapk10 expression was regulated by miR27a-3p in nasopharyngeal carcinoma.Mapk10 gene was down-regulated in the nasopharyngeal carcinoma cells. PMID: 28393229
    2. Peptide mini-scaffold facilitates JNK3 activation in cells. PMID: 26868142
    3. Study found that JNK3 levels are increased in brain tissue and CSF from patients with Alzheimer disease and CSF levels could reflect the rate of cognitive decline PMID: 25455349
    4. Data indicate that tetra-substituted pyridinylimidazoles were designed as dual inhibitors of c-Jun N-terminal kinase (JNK) 3 and p38alpha mitogen-activated protein (MAP) kinase, and both kinases may be involved in the progression of Huntington's disease. PMID: 25475894
    5. JNK3 is required for the antiapoptotic effects of exendin 4 PMID: 25025079
    6. analysis of the unique mechanisms by which JNK1beta1 is regulated PMID: 25178256
    7. Mitogen-activated protein kinase 10 JNK3 alpha (JNK3apha2)binds to both domains of arrestin-3. PMID: 24412749
    8. miR-29b mRNA, MAPK10 protein expression, and ATG9A protein expression are closely related to chemosensitivity of ovarian carcinoma. PMID: 24767251
    9. Subtle structural mechanisms for allosteric signaling between the peptide-binding site and activation loop of human JNK3. PMID: 23142346
    10. reduced JNK3 activity has potentially deleterious effects on neuronal function via altered regulation of a set of post-synaptic proteins. PMID: 23329067
    11. Silent scaffolds: inhibition OF c-Jun N-terminal kinase 3 activity in cell by dominant-negative arrestin-3 mutant. PMID: 22523077
    12. [review] This review focuses on delineating the role of scaffold proteins, especially that of JNK3 as a target, on the regulation of JNK signaling in neurons. PMID: 21321401
    13. Arrestin-3 acts as a "true" scaffold, facilitating JNK3alpha2 phosphorylation by bringing it and MAP kinase kinase (MKK)4 together. PMID: 22047447
    14. The results suggest the possible involvement of CaMKII and JNK3 in soman-induced long-term neurotoxicity. PMID: 21041242
    15. results suggest that MAPK10 may have a proapoptotic function and could function as a tumor-suppressor gene in chromophobe renal cell carcinoma PMID: 21166945
    16. Shares a promoter region with the tightly linked gene encoding Fas-associated phosphatase-1. PMID: 12436199
    17. interaction of free arrestins with JNK3 and Mdm2 and their ability to regulate subcellular localization of these proteins may play an important role in the survival of photoreceptors and other neurons PMID: 16737965
    18. Arrestin in all conformations binds JNK3 comparably, whereas Mdm2 preferentially binds cone arrestin 'frozen' in the basal state. PMID: 17680991
    19. JNK3 recruits MKK4 to the beta-arrestin-2 scaffold complex by binding to the MAPK docking domain (D-domain) located within the N terminus of MKK4. PMID: 18408005

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  • 相关疾病：
    A chromosomal aberration involving MAPK10 has been found in a single patient with pharmacoresistant epileptic encephalopathy. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
  • 亚细胞定位：
    Cytoplasm. Membrane; Lipid-anchor. Nucleus. Mitochondrion.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 组织特异性：
    Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
  • 数据库链接：

    HGNC: 6872

    OMIM: 602897

    KEGG: hsa:5602

    STRING: 9606.ENSP00000352157

    UniGene: Hs.125503

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