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  4. OCLN Recombinant Monoclonal Antibody

OCLN Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    OCLN重组抗体
  • 货号：
    CSB-RA293053A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA293053A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing HepG2 cells surface stained with CSB-RA293053A0HU (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    OCLN Recombinant Monoclonal Antibody（CSB-RA293053A0HU）是一款针对紧密连接蛋白Occludin（OCLN）的高特异性抗体，适用于ELISA、免疫组化（IHC）和流式细胞术（FC）检测。OCLN是维持上皮和内皮细胞紧密连接的关键跨膜蛋白，通过调控细胞旁通透性和细胞极性参与屏障功能，其表达异常与感染、炎症及肿瘤微环境改变密切相关。本抗体经重组表达技术制备，具有高批次一致性，在IHC实验中可清晰定位OCLN蛋白于细胞膜连接处，推荐使用1:50-1:200稀释比例；FC检测中能有效识别细胞表面及胞内OCLN抗原，建议采用相同稀释范围。该产品适用于研究上皮/内皮屏障功能调控机制，例如病原体入侵对紧密连接的破坏、炎症微环境中细胞连接完整性的动态变化，以及肿瘤转移过程中细胞极性相关信号通路分析。科研人员可将其应用于体外细胞模型、组织切片或原代细胞样本的OCLN蛋白定量及亚细胞定位研究，为探索细胞间连接相关疾病机制提供可靠工具。
  • Uniprot No.：
    Q16625
  • 基因名：
    OCLN
  • 别名：
    Occludin, OCLN
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human OCLN
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    8H8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Immunohistochemistry (IHC) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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靶点详情

  • 功能：
    May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.; (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes.
  • 基因功能参考文献：
    1. our findings for the first time identify the role of occludin as a tumor promoter and a prometastatic factor in lung cancer, demonstrating that occludin is a potential prognostic biomarker and therapeutic target in lung cancer PMID: 29750300
    2. Occludin protein may contribute to the development of cervical cancer. However, it was not correlated with the clinical features. PMID: 29516973
    3. Low occludin expression is associated with Renal cell carcinoma. PMID: 28534944
    4. decreased interaction between ZO-1 and occludin might contribute to the epiphora occurred in the transplanted submandibular glands PMID: 28332063
    5. integration of claudin-2, occludin and ZO-1 is necessary for maintaining the function of the proximal tubular epithelium. PMID: 29252987
    6. Molecular studies of the OCLN gene (NM_001205254) identified six distinct candidate mutations in polymicrogyria. PMID: 28179633
    7. Glutamine increased claudin-1 expression in the colonic mucosa of patients with irritable bowel syndrome. In contrast, occludin expression was not significantly modified by glutamine. PMID: 25972430
    8. Endothelial cellsTLR4 strongly regulates retinal vessel permeability by reducing expression of occludin and zonula occludens 1. PMID: 29136627
    9. Suggest endothelial progenitor cell occluding regulates tube formation, sprouting, and proliferation. PMID: 28681912
    10. Viral infectivity was significantly reduced by miR-200c but enhanced by miR-122. PMID: 28642978
    11. The mechanism of transition from nondifferentiated to differentiated states in HepaRG cells was studed by proteomics. Two key factors (MMP-14 and OCLN) were validated by qRT-PCR and Western blot. PMID: 27790907
    12. Intracellular zinc has an essential role in the maintenance of the intestinal epithelial tight junction barrier through regulation of occludin proteolysis and claudin-3 transcription. PMID: 27151944
    13. Data suggest that long noncoding RNA PlncRNA1 and microRNA miR-34c bound together to regulate the expressions of MAZ, ZO-1 and occludin. PMID: 28153728
    14. Study provides evidence that occludin contributes to the regulation of size-reductive proliferation and epithelial cell maturation in a phosphorylation-dependent manner. PMID: 27185880
    15. Report shows that silencing MARCH3 protects the endothelial barrier and upregulates OCLN in MARCH3-depleted cells. MARCH3 silencing results in the strengthening of cell-cell contacts and inactivates FoxO1. PMID: 27616439
    16. STAT3 activation downregulates the ZO-1 and occludin levels and increases the endothelial permeability through the induction of VEGF production in retinal endothelial cells. PMID: 27580405
    17. Downregulation of OCLN is associated with clear cell renal cell carcinoma. PMID: 28184927
    18. These data suggest that the Rho/ROCK signaling pathway is involved in HIV-1 Tat-mediated changes in occludin, RAGE, and LRP1 in human cerebral microvascular endothelial cells cells. PMID: 27563375
    19. OCLN is essential for HCV infection of human hepatic cells. PMID: 26887345
    20. OCLN and ZO1 levels appear to be early prognostic markers in patients suffering from sepsis. PMID: 26863122
    21. autotypic tight junctions molecular composition, like claudin-1 and occludin expression could influence the demyelinating process by altering the permeability of the blood-nerve barrier. PMID: 26662145
    22. Occludin expression has a clear relationship with bone metastasis in human cancer. PMID: 26977027
    23. This is report of a family of Indian origin with two affected sibs and segregation of a homozygous novel OCLN mutation in the exon 3(NG_028291.1(OCLN_v001):c.252delC). PMID: 26689621
    24. Data show that estrogen mediates control of hepatitis C virus through the G-protein-coupled estrogen receptor 30 (GPR30) pathway leading to cleavage of occludin by Matrix Metalloproteinase-9 (MMP-9). PMID: 26731262
    25. In hepatitis C virus -infected human livers, occludin and ADRP mRNA expression levels correlated with each other. Hepatitis C virus increases occludin expression via the upregulation of ADRP. PMID: 26731658
    26. Data show that claudin-6 (CLDN6) R209Q and occludin (OCLN) P24A mutations do not affect HCV pseudoparticles (HCVpp) entry. PMID: 26561856
    27. resistance to HCV in HIV+ patients may be related to genetic variation in SCARB1 or OCLN PMID: 26571379
    28. Angiopoietin-1 Regulates Brain Endothelial Permeability through PTPN-2 Mediated Tyrosine Dephosphorylation of Occludin PMID: 26090670
    29. TIMP1 downregulates MMP-2 activity and inhibits the degradation of occluding in Caco-2 intestinal cells PMID: 25665057
    30. Study uncovers a novel antiviral effect of miR-122 on human liver cells and shows that over-expression of miR-122 can decrease HCV entry into hepatocytes through down-regulation of OCLN. PMID: 25302477
    31. Lipid raft-associated processes, such as PP2A and MMP-2 activation, participate in PCB153-induced disruption of occludin function in brain endothelial barrier. PMID: 26080028
    32. miR-18a and RUNX1 could reversely regulate the permeability of blood-tumor barrier as well as the expressions and distributions of ZO-1, occludin and claudin-5. PMID: 25452107
    33. Zonula occludens-1, occludin and E-cadherin expression and organization in salivary glands PMID: 25248927
    34. LL-37 selectively increased the expression of several claudins and occludin, and enhanced their membrane distribution. PMID: 24862212
    35. In conclusion, our present study indicated that miR-34c regulated the permeability of BTB via MAZ-mediated expression changes of ZO-1, occludin, and claudin-5. PMID: 25201524
    36. findings suggest that cerebral vascular cells express functional BACE1. Moreover, elevated vascular BACE1 may contribute to deficiency of occludin in caa. PMID: 24739782
    37. luciferase assays and chromatin immunoprecipitation assays showed that KLF4 up-regulated the promoter activities and interacted with "CACCC" DNA sequence presented in the promoters of ZO-1, occludin, and claudin-5. PMID: 24318462
    38. We conclude that OCEL-mediated occludin interactions are essential for limiting paracellular macromolecular flux. PMID: 23924897
    39. Data suggest components of diet supplements (here, glutamine/arginine) can improve permeability and tight junction protein expression (OCLN/zona occludens 1) in enterocytes exposed to deleterious effects of antineoplastic agents (here, methotrexate). PMID: 23428392
    40. Eosinophilic esophagitis (EoE), an inflammatory atopic disease of the esophagus, causes massive eosinophil infiltration, basal cell hyperplasia, and sub-epithelial fibrosis. Western analysis of pinch biopsies from EoE and normal pediatric patients indicated reduced expression of intercellular junction proteins, E-cadherin and claudin-1 and increased expression of occludin and vimentin. PMID: 23792687
    41. actin cytoskeletal dynamics is detrimental to METH-induced BBB dysfunction by increasing internalization of occludin. PMID: 24081143
    42. identification of a novel deletion/rearrangement of OCLN that results in band-like brain calcification, limited cognitive abilities, failure to thrive and renal dysfunction in early childhood PMID: 23793442
    43. In HepG2 cells, both hepatitis C virus cell entry and tight junction formation were impaired by OCLN silencing and restored by expression of antibody regulatable OCLN mutant. PMID: 23555257
    44. Loss of occludin is involved in adhesion, apoptosis, differentiation, Ca2+-homeostasis and neoplastic transformation in UV-exposed human keratinocytes and in skin neoplasms. PMID: 23390516
    45. Sinonasal epithelium in allergic fungal rhinosinusitis displays increased epithelial permeability and an altered expression of occludin. PMID: 22927233
    46. HuR promotes occludin translation by blocking occludin mRNA translocation to P-bodies via the displacement of CUGBP1. PMID: 23155001
    47. It is concluded that attenuated expression of the TJ proteins occludin and ZO-1 in human gastric epithelial cells could be involved in clopidogrel-induced gastric mucosal injury through activation of the p38 MAPK pathway PMID: 23220562
    48. Elevated occludin expression is associated with active ulcerative colitis. PMID: 22134947
    49. TTF-1 transcriptionally regulates occludin PMID: 22761434
    50. We define the interface between the ZO-1 PDZ3-SH3-U5-GuK (PSG) and occludin coiled-coil (CC) domains. PMID: 22711802

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  • 相关疾病：
    Pseudo-TORCH syndrome 1 (PTORCH1)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, tight junction.
  • 蛋白家族：
    ELL/occludin family
  • 组织特异性：
    Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
  • 数据库链接：

    HGNC: 8104

    OMIM: 251290

    KEGG: hsa:100506658

    STRING: 9606.ENSP00000347379

    UniGene: Hs.592605

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