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SLAMF7 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    SLAMF7重组抗体
  • 货号：
    CSB-RA021369MA1HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA021369MA1HU diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing A549 cells surface stained with CSB-RA021369MA1HU (red line) at 1:200. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,023 events was performed.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA021369MA1HU SLAMF7重组单克隆抗体是针对CD319/CRACC靶点研发的高特异性检测工具。该抗体识别的SLAMF7蛋白是免疫调控关键分子，在浆细胞、NK细胞及特定T细胞亚群中参与细胞活化信号传导和免疫稳态调节。经多种平台验证，本品在ELISA实验中显示优异抗原结合能力，在免疫组化（IHC）应用中能清晰定位组织切片中的靶蛋白表达，推荐工作浓度1:20-1:200；流式检测（FC）验证显示其可精准标记细胞表面SLAMF7分子，建议使用浓度1:20-1:200。该抗体采用重组技术制备，具有高批次间重复性和低交叉反应性，适用于免疫细胞功能研究、肿瘤免疫微环境分析以及多发性骨髓瘤等疾病模型中免疫检查点机制的探索。在实验设计中，可用于体外评估免疫治疗药物对效应细胞的作用，或通过组织染色解析疾病相关的免疫细胞浸润特征。本产品严格限于科学研究领域，为免疫调控机制和肿瘤免疫治疗基础研究提供可靠检测方案。
  • Uniprot No.：
    Q9NQ25
  • 基因名：
    SLAMF7
  • 别名：
    SLAMF7 antibody; CS1 antibody; UNQ576/PRO1138 antibody; SLAM family member 7 antibody; CD2 subset 1 antibody; CD2-like receptor-activating cytotoxic cells antibody; CRACC antibody; Membrane protein FOAP-12 antibody; Novel Ly9 antibody; Protein 19A antibody; CD antigen CD319 antibody
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human SLAMF7 protein
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Mouse IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    7H1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    FC 1:20-1:200
  • Protocols：
    ELISA Protocol
    Immunohistochemistry (IHC) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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靶点详情

  • 功能：
    Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. P...显示更多
  • 基因功能参考文献：
    1. Memory CD8+ T cells from SLE patients displayed decreased amounts of SLAMF7, a surface receptor that characterizes effector CD8+ T cells. Ligation of SLAMF7 increased CD8+ T cell degranulation capacity and the percentage of IFNgamma-producing cells in response to antigen challenge in SLE patients and healthy controls. SLAMF7 engagement promoted cytotoxic lysis of target cells in response to stimulation with viral antig... PMID: 28076903
    2. phagocytosis of haematopoietic tumour cells during SIRPalpha-CD47 blockade was strictly dependent on SLAM family receptors in vitro and in vivo; in both mouse and human cells, this function required a single SLAM family member, SLAMF7 (also known as CRACC, CS1, CD319), expressed on macrophages and tumour cell targets PMID: 28424516
    3. Cohort statistics revealed a significant increase of circulating sSLAMF7 in multiple myeloma patients versus normal controls PMID: 27116021
    4. Blimp-1 regulates the transcription of CS1 gene in NK and B cell lines from multiple myeloma and diffuse large B cell lymphoma patients. PMID: 26310579
    5. SLAMF7-triggered inhibition is mediated by a mechanism involving Src kinases, CD45, and SHIP-1 that is defective in MM cells PMID: 25312647
    6. Our data highlight the therapeutic potential of targeting CD319 in rheumatoid arthritis PMID: 24299175
    7. SLAMF7 plays an inhibitory role in human monocytes to control proinflammatory immune responses. PMID: 23695528
    8. These results suggest a role for CD319 and CD229 in the systemic lupus erythematosus disease process. PMID: 23956418
    9. These data suggest an involvement of CRACC-mediated NK cell activation in periodontal tissue destruction and point to a plausible distinction in the pathobiology of aggressive and chronic periodontitis. PMID: 23250953
    10. CS1-L and CS1-S may differentially regulate human NK cell functions PMID: 15368295
    11. CS1 may play a role in the regulation of B lymphocyte proliferation during immune responses PMID: 17878365
    12. CS1 was expressed at adhesion-promoting uropod membranes of polarized Multiple Myeloma cells, andis required for MM cell adhesion to bone marrow stromal cells PMID: 17906076
    13. HuLuc63 eliminates myeloma cells, at least in part, via NK-mediated ADCC and shows the therapeutic potential of targeting CS1 with HuLuc63 for the treatment of multiple myeloma. PMID: 18451245

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. Expressed
  • 数据库链接：

    HGNC: 21394

    OMIM: 606625

    KEGG: hsa:57823

    STRING: 9606.ENSP00000357022

    UniGene: Hs.517265

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