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  4. USP24 Recombinant Monoclonal Antibody

USP24 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    USP24重组抗体
  • 货号：
    CSB-RA178866A0HU
  • 规格：
    ￥1320
  • 图片：
    • Immunofluorescence staining of HepG2 cell with CSB-RA178866A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 569-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA178866A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：
    CSB-RA178866A0HU USP24重组单克隆抗体是针对泛素特异性蛋白酶24（USP24）研发的高特异性科研工具。USP24是去泛素化酶家族成员，通过调控靶蛋白稳定性参与DNA损伤应答、细胞周期调控及凋亡等过程，其异常表达与肿瘤发生、神经退行性疾病等病理机制相关。本抗体经ELISA、免疫荧光（IF）和流式细胞术（FC）严格验证，在IF实验中可清晰呈现细胞内USP24的亚定位分布，FC分析能准确检测蛋白表达水平，推荐使用稀释度均为1:50-1:200。其高亲和力与低交叉反应性设计确保实验数据的可靠性，适用于泛素化修饰调控机制研究、疾病相关蛋白降解通路探索及药物靶点筛选等科研场景，为体外细胞模型或组织样本中USP24的功能研究提供稳定支持。该产品适用于基础分子生物学研究与疾病机制解析，不涉及其他用途。
  • Uniprot No.：
    Q9UPU5
  • 基因名：
    USP24
  • 别名：
    Ubiquitin carboxyl-terminal hydrolase 24 (EC 3.4.19.12) (Deubiquitinating enzyme 24) (Ubiquitin thioesterase 24) (Ubiquitin-specific-processing protease 24), USP24, KIAA1057
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human USP24
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    11E3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

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USP24 antibodies

USP24 Proteins

靶点详情

  • 功能：
    Ubiquitin-specific protease that regulates cell survival in various contexts through modulating the protein stability of some of its substrates including DDB2, MCL1 or TP53. Plays a positive role on ferritinophagy where ferritin is degraded in lysosomes and releases free iron.
  • 基因功能参考文献：
    1. USP24 level was decreased during the early stage of cancer and the mitotic stage of the cell cycle to regulate its substrates p300, Bax, E2F4 and securin, resulting in decreased cell apoptosis and increased cell cycle progression and, thus, cancer formation. PMID: 27991932
    2. Data show that ubiquitin-specific peptidase 24 (USP24) is highly expressed in cell lines with enhanced malignancy and in late-stage lung cancer clinical samples. PMID: 26568301
    3. USP24 deubiquitinase regulates the DNA damage response by directly targeting the p53 tumor suppressor. PMID: 25578727
    4. the novel compound EOAI3402143 dose-dependently inhibited Usp9x and Usp24 activity, increased tumor cell apoptosis, and fully blocked or regressed myeloma tumors in mice. PMID: 25814533
    5. These results suggested that USP24 expression is tightly regulated at its transcription level and NFkappaB plays an important role in this process. PMID: 24286619
    6. The knockdown of USP24 in two human cell lines decreased the steady-state levels of DDB2, indicating that USP24-mediated DDB2 deubiquitination prevents DDB2 degradation. PMID: 23159851
    7. The present study is the first to report a lack of association between SNPs of USP24 and parkinson disease in Han Chinese patients. Other PMID: 22923019
    8. USP24 alone plays a role in PD susceptibility among Taiwanese people >or=60 years of age, or acting synergistically with USP40 and UCHL1 in the total subjects. PMID: 20302855
    9. Data suggest that genetic variants in USP24 affect the risk for late-onset Parkinson disease (PD), which is consistent with the predicted role of the ubiquitination pathway in PD etiology. PMID: 16917932

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  • 蛋白家族：
    Peptidase C19 family
  • 数据库链接：

    HGNC: 12623

    OMIM: 610569

    KEGG: hsa:23358

    STRING: 9606.ENSP00000294383

    UniGene: Hs.477009

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