BMI1 Recombinant Monoclonal Antibody
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中文名称：
BMI1重组抗体
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货号：
CSB-RA916472A0HU
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规格：
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图片：
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Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, U87 whole cell lysate, U251 whole cell lysate
All lanes: BMI1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa
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IHC image of CSB-RA916472A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-RA916472A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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其他：
产品详情
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产品描述：
CSB-RA916472A0HU重组单克隆抗体是针对BMI1蛋白研发的高特异性科研试剂，经严格验证适用于ELISA、Western Blot及免疫组化等实验。该抗体靶向BMI1蛋白，该蛋白作为Polycomb抑制复合体PRC1的核心组分，在表观遗传调控中发挥关键作用，参与干细胞自我更新、细胞分化及肿瘤发生等重要生物学过程。经多平台验证数据显示，本产品在Western Blot实验中可清晰识别目标蛋白条带，推荐使用稀释比例为1:500-1:5000；免疫组化应用时能在石蜡包埋组织中准确定位BMI1的亚细胞分布，推荐工作浓度为1:50-1:200。适用于肿瘤生物学领域探索癌干细胞特性、肿瘤微环境调控等研究，也可用于发育生物学中细胞命运决定机制的分析，以及表观遗传学领域内染色质修饰相关研究。本产品采用重组表达技术生产，具有批次间稳定性高、背景信号低的特点，可为研究人员提供可靠的实验数据支持。
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Uniprot No.：
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基因名：
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别名：
Polycomb complex protein BMI-1 (Polycomb group RING finger protein 4) (RING finger protein 51), BMI1, PCGF4 RNF51
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反应种属：
Human
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免疫原：
A synthesized peptide derived from human Bmi1
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免疫原种属：
Homo sapiens (Human)
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标记方式：
Non-conjugated
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克隆类型：
Monoclonal
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抗体亚型：
Rabbit IgG
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纯化方式：
Affinity-chromatography
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克隆号：
1F2
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, IHC
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推荐稀释比：
| Application |
Recommended Dilution |
| WB |
1:500-1:5000 |
| IHC |
1:50-1:200 |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
靶点详情
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功能：
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase acti...
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亚细胞定位：
Nucleus. Cytoplasm.
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数据库链接：
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