HSP90AA1/HSP90AB1 Recombinant Monoclonal Antibody
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中文名称：
HSP90AA1/HSP90AB1重组抗体
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货号：
CSB-RA010802A0HU
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规格：
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图片：
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Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
All lanes: Hsp90 alpha + beta antibody at 1.25μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 90 KDa
Observed band size: 90 KDa
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Immunoprecipitating Hsp90 alpha + beta in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA010802A0HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA010802A0HU (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
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Overlay histogram showing Jurkat cells stained with CSB-RA010802A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
产品详情
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产品描述：
CSB-RA010802A0HU重组单克隆抗体是针对HSP90AA1/HSP90AB1靶点开发的高特异性试剂，适用于多种实验场景。HSP90AA1和HSP90AB1是热休克蛋白90家族成员，作为关键分子伴侣参与蛋白折叠、细胞应激响应及信号转导调控，其功能异常与肿瘤发生发展、神经退行性病变及炎症反应等病理过程密切相关。本抗体经严格验证，可在ELISA、Western Blot（WB）、流式细胞术（FC）和免疫沉淀（IP）中表现优异，推荐使用稀释度为WB 1:500-1:5000、IP 1:200-1:1000，具有批次间稳定性和低交叉反应性特点。实验数据显示，该抗体能特异性识别目标蛋白，在细胞裂解液及多种组织样本中均展现出清晰的检测信号，适用于探究HSP90在蛋白质稳态调控、肿瘤微环境调节或药物靶点筛选等领域的分子机制。其应用场景涵盖基础研究中的蛋白定位分析、相互作用组学研究以及疾病相关信号通路的功能验证，为科研人员提供可靠的实验工具。本产品仅限科学研究使用。
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Uniprot No.：
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反应种属：
Human, Rat
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免疫原：
A synthesized peptide derived from human HSP90AA1/HSP90AB1
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免疫原种属：
Homo sapiens (Human)
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标记方式：
Non-conjugated
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克隆类型：
Monoclonal
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抗体亚型：
Rabbit IgG
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纯化方式：
Affinity-chromatography
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克隆号：
10D6
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, FC, IP
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推荐稀释比：
| Application |
Recommended Dilution |
| WB |
1:500-1:5000 |
| IP |
1:200-1:1000 |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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