SAE1 Recombinant Monoclonal Antibody
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中文名称：
SAE1重组抗体
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货号：
CSB-RA987582A0HU
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规格：
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图片：
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Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: SAE1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 30, 34 kDa
Observed band size: 39 kDa
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Immunofluorescence staining of Hela Cells with CSB-RA987582A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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其他：
产品详情
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产品描述：
CSB-RA987582A0HU SAE1重组单克隆抗体是针对SUMO激活酶复合物关键亚基SAE1研发的高特异性检测工具。该抗体靶向的SAE1蛋白参与调控SUMO化修饰过程，这一翻译后修饰机制在细胞周期进程、DNA损伤修复及蛋白定位等基础生物学过程中发挥重要作用。经多种技术平台验证，本产品在Western Blot（WB）和免疫荧光（IF）实验中展现出优异性能，推荐使用浓度分别为1:500-1:5000和1:20-1:200，其高亲和力可清晰识别内源性SAE1蛋白表达。在ELISA体系中同样具备稳定检测能力，适用于多种实验体系的需求。科研人员可利用该抗体深入探索SUMO化修饰通路在肿瘤发生、神经退行性疾病等病理生理模型中的作用机制，为蛋白质翻译后修饰研究、细胞应激反应分析及药物靶点筛选提供可靠工具。产品严格通过质控验证，确保批次间稳定性，满足细胞生物学、分子机制研究等领域的实验需求。
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Uniprot No.：
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基因名：
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别名：
SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed], SAE1, AOS1 SUA1 UBLE1A
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反应种属：
Human
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免疫原：
A synthesized peptide derived from human SAE1
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免疫原种属：
Homo sapiens (Human)
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标记方式：
Non-conjugated
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克隆类型：
Monoclonal
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抗体亚型：
Rabbit IgG
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纯化方式：
Affinity-chromatography
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克隆号：
9D8
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, IF
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推荐稀释比：
| Application |
Recommended Dilution |
| WB |
1:500-1:5000 |
| IF |
1:20-1:200 |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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