HSU24186 ; MGC120333 ; MGC120334; p30; Replication factor A protein 4; Replication protein A 30 kDa subunit; RF A ; RF-A protein 4; RFA ; RFA4_HUMAN; RP A; RP-A p30; RPA; RPA4
种属：
Homo sapiens (Human)
蛋白长度：
Partial
来源：
E.coli
分子量：
32.8kDa
表达区域：
1-260aa
氨基酸序列
MSKSGFGSYGSISAADGASGGSDQLCERDATPAIKTQRPKVRIQDVVPCNVNQLLSSTVFDPVFKVRGIIVSQVSIVGVIRGAEKASNHICYKIDDMTAKPIEARQWFGREKVKQVTPLSVGVYVKVFGILKCPTGTKSLEVLKIHVLEDMNEFTVHILETVNAHMMLDKARRDTTVESVPVSPSEVNDAGDNDESHRNFIQDEVLRLIHECPHQEGKSIHELRAQLCDLSVKAIKEAIDYLTVEGHIYPTVDREHFKSA Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：
N-terminal 6xHis-tagged
产品提供形式：
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
储存条件：
Store at -20°C/-80°C upon receipt, aliquoting is necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保质期：
The shelf life is related to many factors, storage state,
buffer ingredients, storage temperature and the stability of the protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
注意事项：
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. ...显示更多
基因功能参考文献：
Findings provide the first direct evidence for the function of alternative form of RPA in human DNA metabolism and support a model for aRPA functioning in chromosome maintenance functions in nonproliferating cells. PMID: 19996105
Results suggest that RPA4 cannot support cell proliferation but can support processes that maintain the genomic integrity of the cell. PMID: 19942684
Hyperphosphorylated replication protein A is preferentially localized to double-stranded break repair and the DNA damage checkpoint complexes in response to DNA damage. PMID: 15929725
亚细胞定位：
Nucleus. Note=Localizes to DNA repair foci after DNA damage.
蛋白家族：
Replication factor A protein 2 family
组织特异性：
Preferentially expressed in placental and colon mucosa. Widely expressed at intermediate or lower levels.
