Recombinant Mouse DNA (cytosine-5)-methyltransferase 1 (Dnmt1), partial

1. MiR-139-5p reduces the osteosarcoma cell migration and invasion by regulating DNMT1 and suppresses osteosarcoma tumor growth in vivo. PMID: 29673587
2. Oct4 is involved in DNA methylation through the regulation of Dnmt1 transcription, especially during the early stages of mouse pre-implantation embryo development. PMID: 30140294
3. knockdown of DNMT1 in cerebellar granule neurons caused upregulation of some, but not all subtypes of MHC-I genes. PMID: 29970123
4. Findings indicate that the steady-state level of DNMT1 protein in cycling cell populations is controlled at the level of transcription and that the interaction of DNMT1 and USP7 is unlikely to play a major role in DNMT1 homeostasis. PMID: 29482658
5. Increased shear stress without a change in flow direction initiates arteriogenic growth; however, it also elicits DNMT1-dependent endothelial cells DNA hypermethylation. In turn, this diminishes mechanosensing, augments shear stress set point, and constrains the ultimate arteriogenic capacity of the vessel. PMID: 29191807
6. Single-cell transcriptome analysis detected Dnmt1 expression in murine migratory GABAergic embryonic preoptic area (POA)-derived cells. Deletion of Dnmt1 in postmitotic immature cells of the POA caused defective migration and severely diminished adult cortical interneuron numbers. DNMT1 preserves the migratory shape in part through negative regulation of Pak6, which stimulates neuritogenesis postmigration. PMID: 29117290
7. The aim of the present study is to investigate spatial and temporal expression levels and subcellular localizations of the DNMT1, DNMT3A, and DNMT3B proteins in the mouse germinal vesicle (GV) and metaphase II (MII) oocytes, and early embryos from 1-cell to blastocyst stages. PMID: 29027601
8. DNMT1 ablation from kidney proximal tubules enhanced cisplatin-induced acute kidney injury in mice, suggesting a renoprotective role of DNA methylation. PMID: 28709638
9. Epigenetic enhancement of the post-replicative DNA mismatch repair of mammalian genomes by a Hemi-(m)CpG-Np95-Dnmt1 axis has been demonstrated for humans and mice. PMID: 27886214
10. We show that expression of Id-1, a negative regulator of myogenesis, is enhanced in Dnmt1-deficient cultures, leading to enhanced transdifferentiation of myoblasts toward the osteogenic lineage. Thus, these studies demonstrate that Dnmt1 influences cellular identity and determines lineage fidelity. PMID: 27752090
11. this study shows that DNMT1 might directly repress p53, at least in part, by binding to the p53 promoter locus PMID: 27488808
12. Knockdown of DNMT3A or DNMT1 protected neurons against mutant Htt-induced toxicity, together demonstrating a requirement for DNMTs in mutant Htt-triggered neuronal death and suggesting a neurodegenerative mechanism based on DNA methylation-mediated transcriptional repression. PMID: 27516062
13. squarefindingssquareidentifysquareasquarerobustsquaresexsquaredifferencesquareinsquareDnmt1squareexpressionsquareinsquarethesquarecentral amygdala squarethatsquareissquaresensitivesquaretosquarebothsquarepsychologicalsquareandsquarechemicalsquarestressors. PMID: 28406523
14. Sp1/NFkappaB p65-Dnmt1 pathway may be exploited as a therapeutic target for protecting against podocyte injury in diabetic nephropathy. PMID: 28318634
15. 2-hydroxyglutarate bound to DNMT1 and stimulated its association with the RIP3 promoter, inducing hypermethylation that reduces RIP3 protein and consequently impaired RIP3-dependent necroptosis. PMID: 28564603
16. The results demonstrated that Islet1 upregulated expression of general control of amino acid biosynthesis protein 5 (Gcn5) and enhanced the binding of Gcn5 to the promoters of GATA binding protein 4 (GATA4) and NK2 homeobox 5 (Nkx2.5). In addition, Islet-1 downregulated DNA methyltransferase (DNMT)1 expression and reduced its binding to the GATA4 promoter. PMID: 28447752
17. Data show that RGS6 loss impairs p53 activation and promotes aberrant accumulation of oncogenic protein DNMT1 in urothelium. PMID: 27713144
18. Data (including data from studies using knockout/transgenic mice) suggest that neuronal Dnmt1 regulates energy homeostasis through pathways controlling energy homeostasis; here, neuronal Dnmt1 deficiency prevents diet-induced obesity, reduces adiposity, reduces food intake, and increases energy expenditure in male mice. PMID: 29145563
19. Deletion of DNmt1 in postnatal forebrain neurons results in anxiolytic and antidepressant-like responses. PMID: 27545441
20. Upon lysolecithin injection in the spinal cord of transgenic mice, study detected defective oligodendrocyte progenitor cells differentiation and inefficient remyelination in the DNA methyltransferase 3a null and DNA methyltransferase 1/DNA methyltransferase 3a null mice. PMID: 28451635
21. Data from studies using mouse embryonic fibroblasts suggest that cell proliferation rate positively correlates with expression of Dnmt1 in G1 phase; global DNA methylation is significantly higher in G1 phase than in G2/M phase; larger methylation differences are observed on promoters of pluripotency-related genes; thus, high cell proliferation rates promote generation of induced pluripotent stem cells. PMID: 28924038
22. Dnmt1 and Ezh2 play distinct roles in the different islet cell types PMID: 28119131
23. we extended this work by using a biotinylation tagging approach to characterize DNMT1 protein complexes in mouse erythroleukemic cells. We identified novel DNMT1 interactions with several hematopoietic transcription factors with essential roles in erythroid differentiation PMID: 27693117
24. The lack of Sirt7 is associated with reduced recruitment of DNMT1 and Sirt1 at rRNA genes leading to hyperacetylation of histones, reduced DNA methylation, fragmentation of the nucleolar structure and loss of rDNA repeats leading to anincreased spontaneous immortalization of primary mouse embryonic fibroblasts. PMID: 28842251
25. The lethal Ogden syndrome-associated mutation of Naa10p disrupts its binding to the imprinting control region of H19 and Dnmt1 recruitment. PMID: 28943313
26. The T1505 is crucial on the DNA methylation activity of DNMT1 through stabilizing its structure during ongoing round of DNA methylation. PMID: 28369487
27. reciprocal regulation between miR-148a/152 and DNMT1 in foam cells PMID: 28472596
28. Mutation of all three Dnmts resulted in global genomic hypomethylation and dramatic reorganization of the photoreceptor and synaptic layers within retina. Transcriptome and proteomic analyses demonstrated enrichment of dysregulated phototransduction and synaptic genes. PMID: 27865785
29. indispensable role of DNMT1-mediated epigenetic regulation in postnatal liver growth and regeneration PMID: 26999257
30. SETDB1 maintains silencing of intracisternal A particle, but in the absence of DNMT1, prolonged binding of NP95 to hemimethylated DNA transiently disrupts SETDB1-dependent H3K9me3 deposition. PMID: 27151458
31. The results of this study showed that DNA Methyltransferase 1 is main role of Development of the Hippocampal Dentate Gyrus in mice. PMID: 27251626
32. three DNA methyltransferases, Dnmt1, Dnmt3a, and Dnmt3b, have been identified. Dnmt3a and Dnmt3b are responsible for establishing DNA methylation patterns produced through their de novo-type DNA methylation activity in implantation stage embryos and during germ cell differentiation. Dnmt3-like (Dnmt3l), which is a member of the Dnmt3 family but does not possess DNA methylation PMID: 27826835
33. While lens epithelial cell survival requires DNMT1, morphologically normal lenses develop in the absence of both DNMT3A and DNMT3B. PMID: 27824296
34. Our results provide insights into the molecular basis of DNMT1 dysfunction in hereditary sensory and autonomic neuropathies with dementia and hearing loss patients and emphasize the importance of the TS domain in the regulation of DNA methylation in pluripotent and differentiating cells PMID: 28334952
35. miR-29a mimic transfection lowered collagen 1alpha1, DNMT1, DNMT3b and SET1A expression in hepatic stellate cells. PMID: 28106784
36. These results demonstrate that Dnmt1 and Dnmt3b cooperate to maintain DNA methylation and genomic integrity in the intestinal epithelium. PMID: 26808831
37. DNMT1 mRNA expression was significantly decreased in the isocortex but significantly increased in the prefrontal following hyperoxia PMID: 26592967
38. Cigarette smoke induces proteosomal-mediated degradation of DNMT1 in embryonic orofacial cells. PMID: 26482727
39. the abundance of BDNF positively correlated and phosphorylated DNMT1 inversely correlated with that of FKBP51 in cells PMID: 26602018
40. we suggest that 14-3-3 is a crucial reader of DNMT1pSer143 that regulates DNA methylation and altered gene expression that contributes to cell invasion PMID: 26553800
41. The replication foci targeting sequence domain, not the proliferating cell nuclear antigen - binding domain, is solely responsible for the replication-coupled DNA methylation. PMID: 26383849
42. The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression...the alterations of genome-wide methylation and DNMTs expression may play an important role in the biological effects of 50 Hz ELF-EMF exposure PMID: 26339596
43. Data suggest Fabp4 is up-regulated and DNA methylation is down-regulated in aorta in hyperhomocysteinemia induced by high-methionine diet; up-regulation of DNA methyltransferase 1 (Dnmt1) promotes DNA methylation and decreases Fabp4 expression. PMID: 26606905
44. Obesity-induced, pro-inflammatory cytokines promote DNMT1 expression and its hypermethylation of the adiponectin promoter, suppressing adiponectin expression and exacerbating insulin resistance. PMID: 26139044
45. Dnmt1 is indispensable for mammary stem cell and mammary cancer stem cell maintenance, and for mammary tumorigenesis. Dnmt1 hypermethylates and downregulates ISL1 in mammary tumors and cancer stem cells. PMID: 25908435
46. The hypermethylation of KLF4 directly mediated by Dnmt1 contributes to the progression of EMT in renal epithelial cells. PMID: 25892014
47. the presence of DNMT1 in adult neural stem/precursor cells is crucial for the survival of newly generated neurons, but is dispensable once they accomplish neuronal differentiation in the adult hippocampus. PMID: 25659757
48. Data suggest that Dnmt1 deficiency leads to increased placental triacylglycerol accumulation, abnormal placental mitochondria form/function, and fetal growth retardation; metabolism in the embryo appears unaffected in other parameters investigated. PMID: 25662615
49. DNMT1 was upregulated in the hippocampus in scopolamine-induced memory impairment. PMID: 25982413
50. The DNMT1 intrinsically disordered domain regulates genomic methylation during development. PMID: 25533200

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Nucleus. Cytoplasm. Note=It is nucleoplasmic through most of the cell cycle and associates with replication foci during S-phase. In germ cells, spermatogonia, preleptotene and leptotene spermatocytes all express high levels of nuclear protein, while the protein is not detected in pachytene spermatocytes, despite the fact they expressed high levels of mRNA. In females, the protein is not detected in non-growing oocytes, in contrast to the growing oocytes. During the growing, the protein is no longer detectable in nuclei but accumulates to very high levels first throughout the cytoplasm. At the time of ovulation, all the protein is cytoplasmic and is actively associated with the oocyte cortex. After fecondation, in the preimplantation embryo, the protein remains cytoplasmic and after implantation, it is exclusively nuclear in all tissue types. Isoform 2 is sequestered in the cytoplasm of maturing oocytes and of preimplantation embryos, except for the 8-cell stage, while isoform 1 is exclusively nuclear.

Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family

Isoform 1 is expressed in embryonic stem cells and in somatic tissues. Isoform 2 is expressed in oocytes, preimplantation embryos, testis and in skeletal muscle during myogenesis.

KEGG: mmu:13433

STRING: 10090.ENSMUSP00000004202

UniGene: Mm.128580