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  4. Recombinant Mouse Dynamin-2 (Dnm2), partial

Recombinant Mouse Dynamin-2 (Dnm2), partial

  • 中文名称：
    小鼠Dnm2重组蛋白
  • 货号：
    CSB-YP007079MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Dnm2重组蛋白
  • 货号：
    CSB-EP007079MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Dnm2重组蛋白
  • 货号：
    CSB-EP007079MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Dnm2重组蛋白
  • 货号：
    CSB-BP007079MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Dnm2重组蛋白
  • 货号：
    CSB-MP007079MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dnm2
  • Uniprot No.：
    P39054
  • 别名：
    Dnm2; Dyn2Dynamin-2; EC 3.6.5.5; Dynamin UDNM
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane.
  • 基因功能参考文献：
    1. DNM2 knockdown via two different strategies can efficiently correct the myopathy due to DNM2 mutations. PMID: 30291191
    2. analysis of epistasis between Mtm1 and Dnm2 reveal possibilities for DNM2 knockdown as a therapeutic approach for X-linked centronuclear myopathy (XLCNM), or myotubular myopathy PMID: 28589938
    3. Excitation-contraction coupling is impaired in the muscle fibres of the Dnm22 (R465W) mutant mice. PMID: 29071728
    4. Inhibition of cortactin or dynamin-2 abrogated the increased virus entry observed in DBN1-deficient cells, suggesting that DBN1 suppresses dynamin-mediated endocytosis via interaction with cortactin. PMID: 28416666
    5. The authors demonstrate that ablation of DNM2 in early spermatogenesis results in germ cell arrest during prophase I of meiosis, subsequent loss of all post-meiotic germ cells and concomitant sterility. These effects become exacerbated with age, and ultimately result in the demise of the spermatogonial stem cells and a Sertoli cell only phenotype. PMID: 27725702
    6. expressed throughout the epididymis, but localized to the Golgi apparatus of the principal cells in the proximal segment and the luminal border of these cells in more distal segments PMID: 28395327
    7. Loss of Dynamin 2 GTPase function results in microcytic anaemia. PMID: 28466468
    8. DNM2 mutations cooperate with Lmo2 T-cell oncogenes by enhancing IL-7 signalling. PMID: 27118408
    9. Genetic disruption of Dyn2 GTPase activity selectively in hepatic stellate cells enhances fibrogenesis. PMID: 27840081
    10. Oxidative stress-induced apoptosis and reactive oxygen species production are attenuated by not only Drp1 inhibition but also Dynamin 2 inhibition, implicating Dynamin 2 as a mediator of oxidative stress in cardiomyocytes. PMID: 27802433
    11. Results show that centronuclear myopathy associated Dyn2 mutants are gain-of-function mutations, and their primary effect in muscle is T-tubule disorganization, which explains the susceptibility of muscle to Dyn2 hyperactivity. PMID: 26199319
    12. Dynamin 2 might participate in the early embryonic development through an actin-based cytokinesis PMID: 25421092
    13. Dynamin 2 has a role in endocytosis and in sustaining T-cell receptor signaling and driving metabolic reprogramming in T lymphocytes PMID: 25831514
    14. DNM2-dependent endocytosis in megakaryocytes regulates megakaryopoiesis, thrombopoiesis, and bone marrow homeostasis. PMID: 25468568
    15. Thus dynamin that was recently found to control late stages of myoblast fusion also controls late stages of macrophage fusion, revealing an intriguing conserved mechanistic motif shared by diverse cell-cell fusion processes. PMID: 25336256
    16. FGF21 has a role in promoting endothelial cell angiogenesis through a dynamin-2 and Rab5 dependent pathway PMID: 24848261
    17. Dynamin 2 regulates spindle migration and polar body extrusion during mouse oocyte meiosis through an actin-based pathway PMID: 24735075
    18. Dynamin 2 deficiency caused impaired egress through regulation of S1PR1 signaling. PMID: 24638168
    19. Dnm2 uncouples VEGF signaling from function and coordinates the endocytic turnover of integrins in a manner that is crucially important for angiogenesis in vitro and in vivo. PMID: 24598168
    20. MTM1 and DNM2 regulate muscle organization and force through a common pathway. PMID: 24569376
    21. study found DNM2 function in skeletal muscle is required for proper development; loss of DNM2 function in skeletal muscles initiates a chain of harmful parallel and serial events, involving dysregulation of lipid drolets and mitochondrial defects within altered muscle fibers, defective NMJs and peripheral nerve degeneration PMID: 23813975
    22. These findings provide new evidence for the participation of the autolysosome in hepatocyte lipid droplet metabolism and demonstrate a novel role for dynamin2 in the function and maturation of an autophagic compartment. PMID: 24145164
    23. analysis of the role of Dnm2 in the cross talk between endosomal and autophagic pathways and evidence a new role of Dnm2-dependent membrane trafficking in autophagy which may be relevant in DNM2-related human diseases PMID: 22369075
    24. Study utilized mouse Dyn2 conditional knockout cells combined with retroviral-mediated reconstitution to mimic heterozygous and homozygous states and characterized cellular phenotypes using quantitative assays for several membrane trafficking events. PMID: 21762456
    25. dynamin II may be involved in the transport of vesicles containing collageneous and non-collageneous proteins for dentin formation in odontoblast, suggesting that it is a good nanomolecule as a candidate to regulate the secretion of collagen PMID: 22103132
    26. DNM2 plays important roles in the maintenance of adult muscle fibers PMID: 21514436
    27. This first mouse model of DNM2-related myopathy shows the crucial role of DNM2 in muscle homeostasis. PMID: 20858595
    28. eNOS-dependent dynamin2 S-nitrosylation and invasion of host cells to cause recurrent urinary tract infections. PMID: 21220511
    29. Endocytosis of murine norovirus 1 into murine macrophages is dependent on dynamin II. PMID: 20375172
    30. Data suggest that the components of the GTPase-GED interface act as an intramolecular signaling module, which we term the bundle signaling element, that can modulate dynamin function in vitro and in vivo. PMID: 19515832
    31. These results suggest that hyperoxia induces caveolin-1-dependent, c-Abl-mediated dynamin 2 phosphorylation required for recruitment of p47(phox) to caveolin-enriched microdomains and subsequent ROS production in lung endothelium. PMID: 19833721
    32. To define how Dyn2 might participate in actin dynamics in livings cells we have expressed green fluorescent protein (GFP)-tagged Dyn2 in cultured cells and observed labeling of comet-like vesicles and macropinosomes. PMID: 11782546
    33. All of these results suggest that the interaction between dynamin II and syndecan-4 is important in mediating focal adhesion and stress-fiber formation. PMID: 15694365
    34. results suggest that amphiphysin 1 and dynamin 2 are involved in TBC formation and spermatid release at Sertoli cells PMID: 17785912
    35. Dnm2 mediates fluid-phase micropinocytosis in epithelial cells. PMID: 18003703
    36. These studies reveal isoform and splice-variant specific functions for Dynamin-2. PMID: 18923138
    37. Data suggest that Src activates a negative-feedback loop by promoting both the binding of dynamin to Pyk2-containing complexes and the dynamin-dependent decrease in Pyk2 Y402 phosphorylation, leading to the dissociation of Src from Pyk2. PMID: 19380485
    38. Dyn2 contributes to the structural integrity of discoid vesicles and to the endocytic activity of umbrella cells . PMID: 19479281

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Cell junction. Membrane, clathrin-coated pit. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Midbody. Cell projection, phagocytic cup. Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
  • 组织特异性：
    Expressed in most tissues during embryonic development, including the peripheral nervous system although no expression is evident in skeletal muscle or heart.
  • 数据库链接：

    KEGG: mmu:13430

    STRING: 10090.ENSMUSP00000133564

    UniGene: Mm.433257

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