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  4. Recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (Pik3cb), partial

Recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (Pik3cb), partial

  • 中文名称：
    小鼠Pik3cb重组蛋白
  • 货号：
    CSB-YP806903MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pik3cb重组蛋白
  • 货号：
    CSB-EP806903MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pik3cb重组蛋白
  • 货号：
    CSB-EP806903MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pik3cb重组蛋白
  • 货号：
    CSB-BP806903MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pik3cb重组蛋白
  • 货号：
    CSB-MP806903MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pik3cb
  • Uniprot No.：
    Q8BTI9
  • 别名：
    Pik3cb; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; EC 2.7.1.153; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G...显示更多
  • 基因功能参考文献：
    1. The crucial role of p110beta and the more subtle role of p110alpha in the production of PIP3 molecular species following platelet stimulation has been demonstrated. PMID: 29902570
    2. these results identify the PI3K-GSK3-SMAD1 axis as a central node integrating multiple signaling networks that govern bone formation and homeostasis. PMID: 26896753
    3. Collectively, these findings provide a mechanistic account of how Rac1-dependent membrane raft localization regulates differential activation of distinct phosphoinositide 3-kinase isoforms and offer insight into why PTEN-deficient cancers depend on p110beta. PMID: 27700986
    4. p110beta transduces GPCR signals in the context of coincident activation by an RTK. The p110beta-Gbetagamma interaction was also required for neutrophils to generate reactive oxygen species in response to the Fcgamma receptor-dependent recognition of immune complexes PMID: 27531651
    5. PI3K signaling plays a modulatory role in the regulation of the transcriptional rhythm of the Dbp gene by targeting BMAL1 and CLOCK. PMID: 27022680
    6. Myeloid neoplasia driven by PTEN loss is dependent on p110beta via p110beta-Rac-positive-feedback loop, and that disruption of this loop may offer a new and effective therapeutic strategy for PTEN-deficient leukaemia. PMID: 26442967
    7. p110beta regulates the action of androgens, the male sex hormones, specifically in the Sertoli cells that surround the developing sperm, without affecting androgen action in other tissues. PMID: 26132308
    8. Combined inhibition of PI3Kbeta and PI3Kgamma reduces fat mass by enhancing alpha-MSH-dependent sympathetic drive, thus perhaps a promising treatment for obesity. PMID: 25406378
    9. These findings reveal a role for p110beta during myogenesis and demonstrate that long-term reduction of skeletal muscle p110beta impairs whole-body glucose tolerance without affecting skeletal muscle size or strength in old mice. PMID: 25605332
    10. Platelet PI3Kbeta and GSK3 regulate thrombus stability at a high shear rate. PMID: 25398937
    11. Pancreas-specific disruption of Pik3ca or Rac1, but not Pik3cb, prevented the development of pancreatic tumors in Kras(G12D/+);Ptf1a(Cre/+) mice. PMID: 25311989
    12. Prostate tumor driven by polyomaviruses MT depends on p110alpha but not p110beta. PMID: 24991009
    13. Both p110alpha and p110beta are involved in Akt signaling by IGF-1, but that it is the p110alpha isoform that is responsible for IGF-1-mediated potentiation of platelet function. PMID: 24903091
    14. Genetic ablation of only 3 out of 4 PI3K p110 beta alleles is sufficient to block the development of skin hamartomas resulting from the complete loss of Pten in mice. PMID: 24131925
    15. PTEN-null tumors appear to inherently depend on p110beta, the p110 isoform reliance of PTEN-deficient tumors may be altered by concurrent mutations that activate p110alpha. PMID: 24737887
    16. p110beta subtype of PI3K, followed by activation of Ser473, may possibly participate in the regulation of macrophages activity by wear particles, ultimately resulting in the TNF-alpha secretion and osteolysis. PMID: 23584990
    17. p110beta participated in CXCL12-mediated neural progenitor cell migration. PMID: 23606281
    18. p110alpha acts as a negative regulator of beta cell exocytosis and insulin secretion, while p110beta is a positive regulator of insulin secretion through a mechanism separate from its catalytic activity PMID: 23568272
    19. Activation of PI3K (p110beta and p85beta), and phospho-AKT (Ser473), participated in the regulation of activating macrophages by wear particles, ultimately resulting in the secretion of TNF-alpha. PMID: 23330804
    20. Study investigated the regulation of the ubiquitous p110beta isoform of PI3K, implicated in G-protein-coupled receptor (GPCR) signaling, PTEN-loss-driven cancers, and thrombocyte function. Unexpectedly, RAS is unable to interact with p110beta, but instead RAC1 and CDC42 from the RHO subfamily of small GTPases bind and activate p110beta via its RAS-binding domain. PMID: 23706742
    21. These results indicate that LPA-stimulated PKD2 phosphorylation requires PKCdelta and non-catalytic actions of PI3K p110beta, and provide new information with respect to GPCR-mediated signal transduction in myoblasts. PMID: 23252604
    22. In mammalian cells, p110beta acts as a molecular sensor for growth factor availability and induces autophagy by activating a Rab5-mediated signaling cascade. PMID: 23434372
    23. Phosphoinositide 3-kinase C2beta regulates RhoA and the actin cytoskeleton through an interaction with Dbl PMID: 22984590
    24. a novel p110beta-based regulatory role in receptor-mediated PI3K activity and identify p110alpha as an important target for treatment of HER2-positive disease. PMID: 22802530
    25. In primary and immortalized mouse fibroblast cell lines, both p110alpha and p110beta controlled steady-state PtdIns(3,4,5)P3 levels and Akt signalling induced by heterozygous PTEN loss. PMID: 22150431
    26. data show that p110beta is essential for the ATP- and statin-induced effects and support a role of nuclear pAkt in cancer development. PMID: 22074824
    27. results suggest that PI3K signaling in LepR PMV neurons is essential for leptin-induced alteration in cellular activity. PMID: 21917798
    28. study shows that p110beta nuclear localization signal and p85beta nuclear export sequence regulate p85beta/p110beta nuclear localization, supporting the idea that nuclear, but not cytoplasmic, p110beta controls cell survival PMID: 21383062
    29. PI3K p110-alpha, and not p110-beta, promotes liver steatosis in mice fed an HFD. PMID: 21464441
    30. Neutrophil NADPH oxidase activation by Aspergillus fumigatus hyphal reactive oxygen species (ROS) responses is dependent on class IA phosphoinositide 3-kinase (PI3K)beta and PI3Kdelta catalytic subunits. PMID: 21257963
    31. lipid kinase p110beta/p85beta elucidates an unusual SH2-domain-mediated inhibitory mechanism PMID: 21362552
    32. Results unveil a previously unknown function for p110-beta as a positive regulator of autophagy in multicellular organisms. PMID: 21059846
    33. activation of the p110beta PI3K isoform by c-Kit is required during spermatogenesis, thus opening the way to new treatments for c-Kit positive testicular cancers PMID: 20053680
    34. In IL3-dependent hemopoietic progenitor cells (which express all four class I PI3K isoforms), genetic inactivation of either p110alpha or p110delta did not affect cell proliferation or survival or sensitize to p110beta or p110gamma inactivation PMID: 20534549
    35. prostate-specific expression of a p110beta allele activated by myristoylation induces prostate intraepithelial neoplasia PMID: 20534477
    36. role of p110beta isoform of PI3K in hypothalamic energy regulation PMID: 19883613
    37. Overall, our results provide new evidence for the roles of p110 isoforms in promoting cellular proliferation and homeostasis, IGF-IR internalization, and in opposing apoptosis. PMID: 19834495
    38. class I PI3Kbeta is a critical sensor of genomic integrity PMID: 20368419
    39. PI3Kbeta is a potential target for antithrombotic drugs. PMID: 20065293
    40. important role for PI3K p110 beta in regulating the avidity of high affinity integrin alpha(IIb)beta(3) receptors, necessary for the cellular transmission of contractile forces PMID: 19940148
    41. Early embryonic lethality in mice deficient in the p110beta catalytic subunit of PI 3-kinase PMID: 11919689
    42. p110beta is the major class I catalytic isoform required for apoptotic cell and Fcgamma receptor-mediated phagocytosis by primary macrophages PMID: 12869549
    43. p85 and p110 subunits of PI3-kinase play a role in NF-kappaB activation through interaction with tyrosine-phosphorylated I(kappa)B-alpha and contribute to tyrosine phosphorylation of p65 NF-kappaB PMID: 12870650
    44. Type Ia phosphoinositide 3-kinase (PI3K) p110beta isoform has a key role in regulating the formation and stability of integrin alpha(IIb)beta(3) adhesion bonds, necessary for shear activation of platelets. PMID: 15834429
    45. PI3Kalpha and -beta present distinct activation requirements and kinetics in G(1) phase, with a selective action of PI3Kalpha at the G(0)/G(1) phase transition PMID: 18285463
    46. p110beta, which shows a much broader tissue distribution than the leukocyte-restricted p110gamma, could thus provide a conduit for GPCR-linked PI3K signaling in the many cell types where p110gamma expression is low or absent. PMID: 18544649
    47. ablation of p110beta in the livers of the resulting mice leads to impaired insulin sensitivity and glucose homeostasis, while having little effect on phosphorylation of Akt PMID: 18594509
    48. p110beta catalytic activity is required for PI3K signaling as well as to sustain long-term insulin signaling. In addition, PIK3CB(K805R) mice were protected in a model of ERBB2-driven tumor development. PMID: 18780892
    49. PI3K isoforms can be differentially regulated in distinct cellular contexts, with the dominant role of the p110delta isoform in Akt phosphorylation and proliferation being lost upon cell immortalisation PMID: 19033389
    50. These results show a selective function of PI3Kbeta in the control of DNA replication. PMID: 19416922

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Interaction with PIK3R2 is required for nuclear localization and export.
  • 蛋白家族：
    PI3/PI4-kinase family
  • 数据库链接：

    KEGG: mmu:74769

    STRING: 10090.ENSMUSP00000035037

    UniGene: Mm.213128

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