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  4. Recombinant Mouse Protein phosphatase 1 regulatory subunit 12A (Ppp1r12a), partial

Recombinant Mouse Protein phosphatase 1 regulatory subunit 12A (Ppp1r12a), partial

  • 中文名称：
    小鼠Ppp1r12a重组蛋白
  • 货号：
    CSB-YP872048MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ppp1r12a重组蛋白
  • 货号：
    CSB-EP872048MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ppp1r12a重组蛋白
  • 货号：
    CSB-EP872048MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ppp1r12a重组蛋白
  • 货号：
    CSB-BP872048MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ppp1r12a重组蛋白
  • 货号：
    CSB-MP872048MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ppp1r12a
  • Uniprot No.：
    Q9DBR7
  • 别名：
    Ppp1r12a; Mypt1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
  • 基因功能参考文献：
    1. The findings define the PI3K/NUAK1/MYPT1/MLCP axis as a critical pathway to regulate actomyosin contractility in endothelial cells, supporting vascular patterning and expansion through the control of cell rearrangement. PMID: 30446640
    2. Mypt1 E24 splice variants tune arterial reactivity and could be worthy targets for lowering vascular resistance in disease states. PMID: 27084390
    3. revealed the presence of two MYPT1 isoforms, full length and variant 2 in human intestinal (Caco-2) epithelial cells and isolated intestinal epithelial cells from mice PMID: 27129938
    4. phosphorylation of MYPT1 T694, but not T852, mediates force maintenance in bladder smooth muscle. PMID: 25433069
    5. Constitutive phosphorylation of myosin phosphatase targeting subunit-1 in smooth muscle PMID: 24835173
    6. Maturation of mesenteric artery smooth muscle involves TRA2beta mediated Mypt1 exon 24 splicing. PMID: 25428883
    7. The role of MYPT1 in vascular smooth muscle was investigated in adult MYPT1 smooth muscle specific knock-out mice. MYPT1 deletion enhanced phosphorylation of myosin regulatory light chain and contractile force in isolated mesenteric arteries. PMID: 24951589
    8. MYPT1 is not essential for smooth muscle function in mice but regulates the Ca(2+) sensitivity of force development and contributes to intestinal phasic contractile phenotype. PMID: 23499953
    9. Tra2beta, by regulating the splicing of Mypt1 E23, sets the sensitivity of smooth muscle to cGMP-mediated relaxation. PMID: 22437831
    10. Organ-specific mechanisms involving MYPT1, M-RIP, and CPI-17 are critical to regulating basal LC20 phosphorylation in gastrointestinal smooth muscles. PMID: 21883701
    11. Findings define a new conserved pathway in which sexual development and pregnancy mediate smooth and striated muscle adaptations through SMTNL1 and MYPT1. PMID: 20634291
    12. a reduction in the protein level of MYPT1 (LZ+) is involved in nitrate tolerance; this may result in part from a proteasome-dependent degradation of MYPT1 (LZ+) PMID: 19939965
    13. Stable transfection of HEK 293 cells with GFP-MYPT1 was obtained. MYPT1 and its N-terminal mutants bound to retinoblastoma protein (Rb), raising the possibility that Rb is implicated in the effects caused by overexpression of MYPT1. PMID: 15999227
    14. Ca(2+)-independent relaxation by urocortin can be attributed to cAMP-mediated increased activity of SMPP-1M which is partly attributable to decrease in inhibitory phosphorylation of MYPT1. PMID: 16574904
    15. Therefore, our results show that preservation of GLUT4 expression prevents enhanced arterial reactivity in hypertension, possibly via effects on myosin phosphatase activity. PMID: 17369465
    16. MYPT1 and CARM1 are substrates of OGT and function to alter OGT substrate specificity in vitro PMID: 18840611

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton, stress fiber.
  • 组织特异性：
    Expressed in striated and vascular smooth muscle, specificcally in type 2a fibers (at protein level). Expression levels are 20-30% higher in developed males than females (at protein level).
  • 数据库链接：

    KEGG: mmu:17931

    STRING: 10090.ENSMUSP00000069257

    UniGene: Mm.422959

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