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  4. Recombinant Mouse Rap guanine nucleotide exchange factor 4 (Rapgef4), partial

Recombinant Mouse Rap guanine nucleotide exchange factor 4 (Rapgef4), partial

  • 中文名称：
    小鼠Rapgef4重组蛋白
  • 货号：
    CSB-YP875346MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rapgef4重组蛋白
  • 货号：
    CSB-EP875346MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rapgef4重组蛋白
  • 货号：
    CSB-EP875346MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rapgef4重组蛋白
  • 货号：
    CSB-BP875346MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rapgef4重组蛋白
  • 货号：
    CSB-MP875346MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rapgef4
  • Uniprot No.：
    Q9EQZ6
  • 别名：
    Rapgef4; Cgef2; Epac2; Rap guanine nucleotide exchange factor 4; Exchange factor directly activated by cAMP 2; Exchange protein directly activated by cAMP 2; EPAC 2; cAMP-dependent Rap1 guanine-nucleotide exchange factor; cAMP-regulated guanine nucleotide exchange factor II; cAMP-GEFII
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
  • 基因功能参考文献：
    1. Epac2(-/-) mice exhibited a wide range of mood disorders, including anxiety and depression with learning and memory deficits in contextual and cued fear-conditioning tests without affecting Epac1 expression or PKA activity. PMID: 27598965
    2. PACAP could trigger astrocytic differentiation of neural stem cells via Epac2 activation. PMID: 26645637
    3. Epac2, but not Epac1 has a role in decreasing severity of ischemic retinopathy after retinal ischemia/reperfusion injury PMID: 25985753
    4. cAMP-GEF II plays a key role in hippocampal functions including behavioral flexibility. PMID: 26104314
    5. data suggest that cAMP elevation uses an Epac2-dependent pathway to promote transmitter release, and that Epac2 is required to maintain the readily releasable pool at Mossy Fiber synapses in the hippocampus. PMID: 25904804
    6. Epac1 primarily is capable of inhibiting remodeling processes, whereas Epac2 primarily increases inflammatory processes in vivo PMID: 25103224
    7. EPAC1 and EPAC2 are critical signaling intermediates in osteoclast differentiation that permit RANKL-stimulated NFkB nuclear translocation and actin rearrangements PMID: 25122553
    8. Data suggest that sulfonylureas stabilize Epac2A in its open, active state and provide insight for the development of drugs that target Epac2A. PMID: 24150255
    9. data show the expression EPAC is altered in the cortex, striatum and midbrain of HPRT knockout mouse; propose that the alterations in EPAC/RAP1 signaling and cell migration in HPRT deficiency are crucial for neuro-developmental events that may contribute to neurological dysfunctions in Lesch-Nyhan syndrome PMID: 23804752
    10. DNA methylation of alternative promoters directs tissue specific expression of Epac2 isoforms. PMID: 23861833
    11. Epac2 localization is dynamically controlled by cAMP as well as by Ca(2+)-mediated activation of Ras. PMID: 23633676
    12. EPAC2A is required for potentiating the early dynamic increase in islet calcium levels after glucose stimulation, which is reflected in potentiated first-phase insulin secretion. PMID: 23578994
    13. there is a gut-heart GLP-1R-dependent and ANP-dependent axis that regulates blood pressure and involves Epac2 PMID: 23542788
    14. beta1-induced adrenergic-dependent arrhythmias are less inducible in Epac2-knockout versus wild-type mice. PMID: 23363625
    15. This study demonistrated that exhibited specific impairments in social and communication behavior displayed specific alterations in cortical columnar organization in the somatosensory cortex. PMID: 22915127
    16. analysis of autoinhibition and activation of the exchange protein directly activated by cyclic AMP (EPAC) and EPAC2 PMID: 21873431
    17. Phospholipase c-epsilon is a determinant of mouse islet insulin secretion that is under the control of Epac2. PMID: 21478675
    18. Phospholipase C-eta was found to be expressed in mouse beta-cells, and knockout of that gene disrupts the action of exendin-4 to facilitate calcium-induced calcium release in the beta-cells of these mice. PMID: 21041529
    19. Piccolo serves as a Ca(2+) sensor in exocytosis in pancreatic beta-cells and the formation of a cAMP-GEFII.Rim2.Piccolo complex is important in cAMP-induced insulin secretion PMID: 12401793
    20. Mutational analysis suggests a model for cAMP-induced Epac activation with rigid body movement of the regulatory region, the features of which are universally conserved in cAMP-regulated proteins. PMID: 16452984
    21. cAMP at least partially regulates proglucagon gene expression via the Epac-Ras/Rap-Raf-MEK-ERK signaling pathway PMID: 16644915
    22. Northern blot analysis and in situ hybridization suggested that Epac1 and Epac2 mRNAs were transcribed in the seminiferous epithelia of the testis PMID: 17077580
    23. These data indicate that Epac2/Rap1 signaling is essential in regulation of insulin granule dynamics by cAMP, most likely by controlling granule density near the plasma membrane. PMID: 18040047
    24. AKAP150 acts as a key regulator in the two cAMP pathways to control PKB/Akt phosphorylation. PMID: 18565730
    25. structure of Epac2 in complex with a cAMP analogue (Sp-cAMPS) and RAP1B by X-ray crystallography and single particle electron microscopy PMID: 18660803
    26. Ras and cAMP regulate Epac2 function in a parallel fashion and the Ras-Epac2 interaction is required for the cAMP-dependent activation of endogenous Rap1 by Epac2. PMID: 18824540
    27. Results are consistent with a cAMP/Epac2/Rap1-mediated signaling that could exert its action, among others, through RA-RhoGAP to promote the progression of spermatogenesis. PMID: 18937323
    28. Cyclic AMP-dependent Epac2 signals to ERK1/2 and Akt upon activation of H-Ras, and is involved in BCR-induced growth arrest and apoptosis in WEHI-231 cells. PMID: 19167486
    29. splicing variant Epac2B, which lacks the first cAMP-binding domain A in the N-terminus but has the second cAMP-binding domain B of Epac2A, possesses GEF activity towards Rap1, as was found for Epac2A PMID: 19170062

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  • 亚细胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein.
  • 组织特异性：
    Expressed in cerebellum, pituitary, adrenal gland and liver.
  • 数据库链接：

    KEGG: mmu:56508

    STRING: 10090.ENSMUSP00000088336

    UniGene: Mm.196153

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