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  4. Recombinant Mouse Serine/threonine-protein kinase ULK1 (Ulk1), partial

Recombinant Mouse Serine/threonine-protein kinase ULK1 (Ulk1), partial

  • 中文名称：
    小鼠Ulk1重组蛋白
  • 货号：
    CSB-YP025612MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ulk1重组蛋白
  • 货号：
    CSB-EP025612MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ulk1重组蛋白
  • 货号：
    CSB-EP025612MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ulk1重组蛋白
  • 货号：
    CSB-BP025612MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ulk1重组蛋白
  • 货号：
    CSB-MP025612MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ulk1
  • Uniprot No.：
    O70405
  • 别名：
    Ulk1Serine/threonine-protein kinase ULK1; EC 2.7.11.1; Serine/threonine-protein kinase Unc51.1; Unc-51-like kinase 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, le...显示更多
  • 基因功能参考文献：
    1. Ulk1-Knockout mice show reduced inflammation and apoptosis in hippocampus after traumatic brain injury. PMID: 29680658
    2. The authors described a long-distance signaling mechanism of Porcine hemagglutinating encephalomyelitis virus-driven deficits in neurons and suggested that such Ulk1 repression may result in limited NGF/TrkA retrograde signaling within activated Rab5 endosomes, explaining the progressive failure of neurite outgrowth and survival. PMID: 29875237
    3. Authors conclude that miR-93 is involved in hypoxia-induced autophagy by regulating ULK1. Results provide a new angle to understand the complicated regulation of the key autophagy kinase ULK1 during different stress conditions. PMID: 29109831
    4. Ampk is required for exercise-induced phosphorylation of Ulk1. PMID: 28916822
    5. We created Smcr8 knockout mice and found that Smcr8 mutant cells exhibit impaired autophagy induction, which is similarly observed in C9orf72 knockdown cells. Mechanistically, SMCR8/C9ORF72 interacts with the key autophagy initiation ULK1 complex and regulates expression and activity of ULK1 PMID: 27617292
    6. ATG7, but not ATG13 or ULK1 has a role in functional autophagy in glioblastoma development PMID: 27304681
    7. ULK1 is a novel substrate of Caspase-3 and upregulation of ULK1 drives autophagy initiation in leukemia cells. PMID: 28381396
    8. Findings indicate that the PPM1D-Ulk1 axis plays a pivotal role in genotoxic stress-induced autophagy. PMID: 27670885
    9. Regulation of endoplasmic reticulum-to-Golgi trafficking by ULK1/2 is essential for cellular homeostasis. PMID: 27203176
    10. Ulk1-mediated autophagy plays an essential role in mitochondrial remodeling and functional regeneration of skeletal muscle. PMID: 28356270
    11. ULK1/2 function as a bifurcate-signaling node that sustains glucose metabolic fluxes besides initiation of autophagy in response to nutritional deprivation. PMID: 27153534
    12. These results define a key molecular event for the starvation-induced activation of the ATG14-containing PtdIns3K complex by ULK1. PMID: 27046250
    13. By enhancing PARP1 activity, ULK1 contributes to ATP depletion and death of H2O2-treated cells. PMID: 26138443
    14. MUL1 ubiquitinates ULK1 and regulates selenite-induced mitophagy PMID: 26018823
    15. autophagy can be executed by mechanisms that are dependent or independent of the ULK1/2-ATG13 interaction. PMID: 26213203
    16. MiR-17-5p may be able to arrest the maturation of mycobacterial phagosomes in part by targeting ULK1, subsequently reduces the ability of host cells to kill intracellular Camette-Guerin bacillus. PMID: 26384021
    17. Study identifies a key role of Cul3-KLHL20 in autophagy termination by controlling autophagy-dependent turnover of ULK1 and VPS34 complex subunits and reveals the pathophysiological functions of this autophagy termination mechanism. PMID: 26687681
    18. Concurrent mTORC1 inactivation and PP2A-B55alpha stimulation fuel ULK1-dependent autophagy. PMID: 26310906
    19. AnxA2 regulates autophagy, thereby contributing to host immunity against bacteria through the Akt1-mTOR-ULK1/2 signaling pathway PMID: 26371245
    20. Nitric oxide also stabilized an autophagy-related protein unc-51 like kinase (ULK1), but did not restore SIRT1 protein levels in ULK1-siRNA-treated cells or in mouse embryonic fibroblasts (MEF) from Ulk1-/- mice. PMID: 25541949
    21. Ulk1-dependent Atg5-independent macroautophagy is the dominant process of mitochondrial clearance from fetal definitive reticulocytes. PMID: 24895007
    22. ULK1 knockdown increased neuronal cell viability, and enhanced S6k1 phosphorylation in a dopaminergic neuron model of Parkinson's disease PMID: 25680463
    23. this study identifies ULK1 as a new p62 Ser403 kinase and establishes Sestrin2 as a promoter of ULK1-mediated p62 phosphorylation. PMID: 25040165
    24. ULK1, albeit its important autophagic role, regulates lipid metabolism and glucose uptake in adipocytes distinctly from other autophagy proteins. PMID: 24135897
    25. Analysis of the autophagy signaling pathway showed the existence of a negative feedback loop between the ULK1 and 2 and MTORC1 and 2, in lung tissue. PMID: 24275123
    26. Data suggest that Ulk1 plays critical role in development of alcoholic cardiomyopathy, that is, autophagy induction, cardiac contractile dysfunction, and intracellular Ca2+ anomalies. PMID: 23448468
    27. ULK1 and ULK2 have redundant roles in nutrient-dependent activation of autophagy PMID: 23291478
    28. ULK1 induces autophagy by phosphorylating Beclin-1 and activating VPS34 lipid kinase. PMID: 23685627
    29. Both AMPK and ULK1 regulate localization of a critical component of the phagophore, ATG9 PMID: 22932492
    30. In the initial steps of Parkin-mediated mitophagy, the structures containing the ULK1 complex and Atg9A are independently recruited to depolarized mitochondria. PMID: 22275429
    31. amino acid starvation regulates autophagy in part through an increase in cellular Ca(2+) that activates a CaMKK-beta-AMPK pathway and inhibits mTORC1, which results in ULK1 stimulation PMID: 23027865
    32. Unexpected and essential role of autophagy during monocyte differentiation and acquisition of macrophage functions. PMID: 22452982
    33. Phosphorylation of AMPK by Ulk1 represents a negative feedback circuit. PMID: 21460634
    34. ULK1 and ULK2 are functionally redundant protein kinases required to mediate autophagy under nutrient-deprived conditions in fibroblasts. PMID: 21460635
    35. the autophagy response to the enhanced amino acid catabolism induced by deprivation of glucose or direct exposure to ammonia does not require ULK1 and/or ULK2 PMID: 21690395
    36. Nutrient starvation elicits an acute autophagic response mediated by Ulk1 dephosphorylation and its subsequent dissociation from AMPK. PMID: 21383122
    37. Data show that under nutrient sufficiency, high mTOR activity prevents Ulk1 activation by phosphorylating Ulk1 Ser 757 and disrupting the interaction between Ulk1 and AMPK. PMID: 21258367
    38. findings uncover a conserved mechanism coupling nutrient status with autophagy and cell survival; loss of AMPK or ULK1 result in defective mitophagy;phosphorylation of ULK1 by AMPK required for mitochondrial homeostasis and cell survival during starvation PMID: 21205641
    39. Results identified multiple sites of phosphorylation on Ulk1, including several within domains known to regulate macroautophagy, and sites in the C-terminal domain that depend upon or require Ulk1 autophosphorylation. PMID: 19807128
    40. Complex of Unc51.1/SynGAP1/Syntenin governs axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons PMID: 15014045
    41. ULK1 is localized to vesicular structures in growth cones of spinal ganglia afferent neurons. RNAi-mediated knockdown of ULK1 resulted in impaired endocytosis of nerve growth factor, excessive axon arborization, and severely stunted axon elongation. PMID: 17389358
    42. Ulk1, is a component of the selective autophagy machinery that leads to the elimination of organelles in erythroid cells rather that an essential mechanistic component of autophagy PMID: 18539900
    43. The functions of ULK1 and ULK2 are controlled by autophosphorylation and conformational changes involving exposure of the C-terminal domain and interaction with the putative human homologue of Atg13. PMID: 18936157
    44. The role of the ULK-FIP200 complex in autophagy and the possibility that FIP200 functions as a mammalian counterpart of Atg17, is reported. PMID: 18981720
    45. mTORC1 suppresses autophagy through direct regulation of the approximately 3-MDa ULK1-Atg13-FIP200 complex. PMID: 19211835
    46. ATG13 and ULK1 are phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner, indicating that the ULK1.ATG13.FIP200 complex acts as a node for integrating incoming autophagy signals into autophagosome biogenesis. PMID: 19258318

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  • 亚细胞定位：
    Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily
  • 数据库链接：

    KEGG: mmu:22241

    STRING: 10090.ENSMUSP00000031490

    UniGene: Mm.271898

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