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  4. Recombinant Mouse Serine/threonine-protein kinase/endoribonuclease IRE1 (Ern1), partial

Recombinant Mouse Serine/threonine-protein kinase/endoribonuclease IRE1 (Ern1), partial

  • 中文名称：
    小鼠Ern1重组蛋白
  • 货号：
    CSB-YP880635MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ern1重组蛋白
  • 货号：
    CSB-EP880635MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ern1重组蛋白
  • 货号：
    CSB-EP880635MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ern1重组蛋白
  • 货号：
    CSB-BP880635MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ern1重组蛋白
  • 货号：
    CSB-MP880635MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ern1
  • Uniprot No.：
    Q9EQY0
  • 别名：
    Ern1; Ire1Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a) [Includes: Serine/threonine-protein kinase; EC 2.7.11.1); Endoribonuclease; EC 3.1.26.-)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease ac...显示更多
  • 基因功能参考文献：
    1. IRE1alpha has a critical role in colonic tumorigenesis and IRE1alpha targeting might be a strategy for treatment of colon cancers. PMID: 28825721
    2. Study provided the first link between energy homeostasis, lipid metabolism and pro-opiomelanocortin (POMC) neuronal IRE1alpha function. The effects of POMC IRE1alpha knockout were mediated via increased thermogenesis, possibly caused by the increased alpha-MSH production in the hypothalamus. These results demonstrate that IRE1alpha in POMC neurons plays a critical role in the regulation of obesity and obesity-related m... PMID: 27558934
    3. cytokine-activated STAT3 and STAT6 cooperate in macrophages to promote a secretory phenotype that enhances tumor progression in a cathepsin-dependent manner. PMID: 27626662
    4. Data suggest that activation of GRP78/Ire1/Xbp1 pathway of ER stress-unfolded protein response is involved in mouse decidualization. PMID: 27283502
    5. Fortilin directly interacts with the cytoplasmic domain of IRE1alpha, inhibits both kinase and endoribonuclease (RNase) activities of this stress sensor, and protects cells against apoptotic cell death at both cellular and whole animal levels. PMID: 28550308
    6. defective autophagy in intestinal epithelial cells (IECs) may predispose to Crohn's disease ileitis via impaired clearance of IRE1alpha aggregates during ER stress at this site. PMID: 28082357
    7. The findings indicate that IRE1-XBP1 downregulation distinguishes germinal center B-cell-like diffuse large B-cell lymphoma (DLBCL) from other DLBCL subtypes and contributes to tumor growth. PMID: 28167662
    8. the ABL family of tyrosine kinases rheostatically enhances IRE1alpha's enzymatic activities, thereby potentiating endoplasmic reticulum stress-induced apoptosis. PMID: 28380378
    9. We reveal distinct binding affinities between the binary and ternary complexes thus formed, that suggest a preference for the PERK signaling branch under stress, and a predilection for the GRP78-UPR sensor complex formation upon stressor removal. These results imply a gated UPR mechanism that tunes the overall cellular behavior to the accumulation of unfolded proteins. PMID: 28416388
    10. Inositol-Requiring Enzyme 1 Facilitates Diabetic Wound Healing Through Modulating MicroRNAs. PMID: 27634225
    11. Ire1alpha-Xbp1s and associated molecular targets link ER stress in arcuate Pomc neurons to aspects of normal energy and glucose homeostasis. PMID: 28028078
    12. heat stress simultaneously activates both the unfolded protein response (UPR) and autophagy, followed by the activation of a negative feedback system in UPR by modulating the responses related to the IRE1alpha-XBP-1 axis. PMID: 27743894
    13. this study shows that IRE1alpha is a critical switch governing M1-M2 macrophage polarization and energy balance in obesity PMID: 28346409
    14. Findings indicate that PERK kinase-activating transcription factor 4 (ATF4) pathway affected the efficiency of X-box binding protein 1 (XBP1) mRNA splicing by regulating inositol-requiring enzyme 1alpha (IRE1alpha) expression. PMID: 27052593
    15. Palmitate increased TRB3 expression, activated IRE1alpha signaling, and reduced the insulin-dependent Akt phosphorylation. Knocking down TRB3 or IRE1alpha did not prevent the inhibitory effect of palmitate on Akt phosphorylation. Results support the idea that ER stress is not responsible for lipid-induced IR in C2C12 myotubes. PMID: 26337904
    16. PKA-dependent IRE1alpha-XBP1 activation is crucial for the transcriptional induction of Ucp1 in brown adipocytes. PMID: 26568450
    17. Nitric oxide can S-nitrosylate the endoplasmic reticulum stress sensors IRE1alapha and PERK. PMID: 26446798
    18. SphK1 inhibited the IRE1alpha pathway by reducing IRE1alpha expression at the transcriptional level. PMID: 26240153
    19. IRE1alpha may promote liver regeneration through acting as a signaling platform to regulate the STAT3 pathway. PMID: 25457211
    20. The present study thus reveals an intracellular pathway that integrates the UPR and osteoclast differentiation through activation of the IRE1alpha/XBP1 pathway. PMID: 26193638
    21. CReP expression was markedly suppressed in XBP1-deficient mice livers due to hyperactivated Ire1alpha. PMID: 26031337
    22. results suggest that IRE1alpha functions to maintain the intestinal epithelial homeostasis and plays an important role in defending against inflammation bowel diseases PMID: 25925952
    23. These results indicate that IRE1alpha can promote beta-cell proliferation through, at least in part, XBP1s upregulation of cyclin D1, which is known to drive cells from the G1 into the S-phase of the cell cycle. PMID: 24797433
    24. these data describe a mechanism by which inflammatory pathways compromise unfolded protein response function through iNOS-mediated S-nitrosylation of IRE1alpha, which contributes to defective IRE1alpha activity, impaired ER function, and prolonged endoplasmic reticulum stress in obesity. PMID: 26228140
    25. SEC63 deficiency selectively activates the IRE1alpha-XBP1 branch of the unfolded protein response. SEC63 function regulates IRE1alpha/XBP1 activation. PMID: 25844898
    26. ER stress-mediated IRE1alpha activation is part of mutant Htt toxicity and that this is counteracted by Usp14 expression. PMID: 24951540
    27. RIPK1 indirectly regulates caspase-8 activation, in part via interaction with the ER stress sensor IRE1. PMID: 25476903
    28. Endoplasmic reticulum stress-induced IRE1alpha activation mediates cross-talk of GSK-3beta and XBP-1 to regulate inflammatory cytokine production. PMID: 25821218
    29. IRE1alpha demonstrates prominent expression in proliferating and prehypertrophic chondrocytes in thewildtype embryonic growth plate, whereas its expression is hardly detectable in GEP-/- embryos. PMID: 24863879
    30. The IRE1alpha-XBP1 pathway of the unfolded protein response could directly activate the transcriptional expression of Fgf21. PMID: 25170079
    31. Data show that hepatic IRE1alpha protein promotes the adaptive shift of fuel utilization during starvation by stimulating mitochondrial beta-oxidation and ketogenesis through the X-box binding protein 1-Peroxisome proliferator-activated receptor alpha axis. PMID: 24670948
    32. IRE1a, BMP2, GEP growth factor, and JunB transcription factor form a regulatory loop and act in concert in the course of osteoblastogenesis. PMID: 24853417
    33. The transcriptional activity of activating transcription factor 6 (ATF6) remained unchanged after incubation with H2O2. PMID: 24915138
    34. a precisely regulated feedback circuit involving IRE-1alpha and XBP-1 controls the homeostasis of CD8alpha+ conventional dendritic cells PMID: 24441789
    35. IRE1 downregulation represents a previously undescribed viral strategy to curb the UPR. PMID: 23950715
    36. IRE1alpha dissociates from BiP and inhibits ER stress-mediated apoptosis in the process of cartilage development. PMID: 23816533
    37. This study identifies a regulatory function for IRE1alpha in the promotion of IL-4 in T cells. PMID: 24100031
    38. A novel regulatory axis through TRAF6-mediated IRE1alpha ubiquitination indicates that IRE1alpha is a potential therapeutic target for inflammatory arthritis. PMID: 23942232
    39. eIF2alpha and CHOP, but not IRE1alpha, have roles in ER stress signaling that attenuates adipogenesis in mice PMID: 23314846
    40. Neuronal ER stress impedes myeloid-cell-induced vascular regeneration through IRE1alpha degradation of netrin-1. PMID: 23473031
    41. Disruption of IRE1alpha and XBP1 drastically attenuated the expression of Cts7 and Cts8, which are essential cathepsins for placenta formation. PMID: 23541586
    42. The IRE1alpha-XBP1 pathway positively regulates parathyroid hormone (PTH)/PTH-related peptide receptor expression and is involved in PTH-induced osteoclastogenesis. PMID: 23235147
    43. An essential role for the inositol-requiring transmembrane kinase/endoribonuclease 1alpha (IRE1alpha)-X box binding protein 1 (XBP1) arm of the UPR is in regulation of hepatic very low-density lipoprotein (VLDL) assembly and secretion. PMID: 23040069
    44. IRE1 alpha degrades mRNAs encoding lipid metabolism regulators. PMID: 23040070
    45. IRE1-alpha activation and signaling require concerted coordination between the endoplasmic reticulum and cytoskeleton PMID: 23237951
    46. IRE1alpha induces thioredoxin-interacting protein to activate the NLRP3 inflammasome and promote programmed cell death under irremediable ER stress. PMID: 22883233
    47. IRE1alpha regulates translation of a proapoptotic protein, Caspase-2, through terminating microRNA biogenesis, and noncoding RNAs are part of the ER stress response PMID: 23042294
    48. The authors show that 1 alpha (IRE1alpha), an ER transmembrane protein kinase/endoribonuclease, is required to maintain hepatic lipid homeostasis under ER stress conditions through repressing hepatic lipid accumulation and maintaining lipoprotein secretion. PMID: 21407177
    49. The authors identified the BCL-2 family member PUMA as a novel IRE1alpha interactor. Immun oprecipitation experiments confirmed this interaction and further detected the association of IRE1alpha with BIM, another BH3-only protein. PMID: 22510886
    50. IRE1alpha plays an essential role in the down-regulation of Cyp1a2 and Cyp2e1 mRNAs under endoplasmic reticulum stress. PMID: 22291093

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 组织特异性：
    Ubiquitously expressed. High levels in thymus, liver and lung. In the brain, preferentially expressed in cortical, hippocampal and olfactory neurons.
  • 数据库链接：

    KEGG: mmu:78943

    STRING: 10090.ENSMUSP00000001059

    UniGene: Mm.340943

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