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  4. Recombinant Yellow fever virus Genome polyprotein, partial, Biotinylated

Recombinant Yellow fever virus Genome polyprotein, partial, Biotinylated

  • 中文名称：
    Recombinant Yellow fever virus Genome polyprotein, partial, Biotinylated
  • 货号：
    CSB-EP365905YAC1g10-B
  • 规格：
    ￥3324
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    N/A
  • Uniprot No.：
    P03314
  • 别名：
    Core protein;Matrix protein;NS1;NS2A;NS2A-alpha;Flavivirin protease NS2B regulatory subunit;Non-structural protein 2B;EC 3.4.21.91;EC 3.6.1.15;EC 3.6.4.13;Flavivirin protease NS3 catalytic subunit;Non-structural protein 3;NS4A;NS4B;EC 2.1.1.56;EC 2.1.1.57;EC 2.7.7.48;Non-structural protein 5
  • 种属：
    Yellow fever virus (strain 17D vaccine) (YFV)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    96.2 kDa
  • 表达区域：
    286-730aa
  • 氨基酸序列
    AHCIGITDRDFIEGVHGGTWVSATLEQDKCVTVMAPDKPSLDISLETVAIDRPAEVRKVCYNAVLTHVKINDKCPSTGEAHLAEENEGDNACKRTYSDRGWGNGCGLFGKGSIVACAKFTCAKSMSLFEVDQTKIQYVIRAQLHVGAKQENWNTDIKTLKFDALSGSQEVEFIGYGKATLECQVQTAVDFGNSYIAEMETESWIVDRQWAQDLTLPWQSGSGGVWREMHHLVEFEPPHAATIRVLALGNQEGSLKTALTGAMRVTKDTNDNNLYKLHGGHVSCRVKLSALTLKGTSYKICTDKMFFVKNPTDTGHGTVVMQVKVSKGAPCRIPVIVADDLTAAINKGILVTVNPIASTNDDEVLIEVNPPFGDSYIIVGRGDSRLTYQWHKEGSSIGKLFTQTMKGVERLAVMGDTAWDFSSAGGFFTSVGKGIHTVFGSAFQGL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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N/A Proteins

靶点详情

  • 功能：
    Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.; Inhibits RNA silencing by interfering with host Dicer.; Prevents premature fusion activity of envelope proteins in trans-Golgi by binding to envelope protein E at pH6.0. After virion release in extrace...显示更多
  • 基因功能参考文献：
    1. adaptive genetic diversification has occurred on viral nonstructural protein 5 in African and South American yellow fever virus populations PMID: 28044043
    2. This suggests that DNAJC14's folding activity normally modulates yellow fever virus NS3/4A/2K cleavage events to liberate appropriate levels of NS3 and NS4A and promote replication complex formation. PMID: 26739057
    3. These data support a complex interplay between yellow fever virus NS2A and NS3 in virion assembly and identify a basic cluster in the NS2A N terminus to be critical in this process.[NS2A, NS3] PMID: 25694595
    4. Binding of Yellow fever virus NS5 to the IFN-activated transcription factor STAT2 only in cells that have been stimulated with IFN-beta. PMID: 25211074
    5. While the increase of the positive charge in the envelope protein domain III may reduce the virulence of YFV in mice, this mutation favored the establishment of the viral infection in Aedes aegypti. PMID: 24678844
    6. These results indicate an interaction of human eIF3L with yellow fever virus NS5 and that eIF3L overexpression facilitates translation, which has potential implications for virus replication. PMID: 23800076
    7. study shows that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation; a region between amino acids 368 and 448 was identified as the site of interaction of the NS5 protein with U1A PMID: 21298455
    8. A combination of several signal peptides contributes to the endoplasmic reticulum-retention of the yellow fever virus envelope protein. PMID: 19846669
    9. These data indicate that certain mutations that reduce NS2B-NS3 protease cleavage activity cause growth restriction of yellow fever virus in cell culture. PMID: 15831952
    10. analysis of the yellow fever virus capsid protein PMID: 17526891
    11. mutation of a conserved tryptophan at position 349 in the helicase domain of NS3 blocks production of infectious virus particles, revealing an as-yet-unknown role for NS3 in virus assembly. PMID: 18199634
    12. The data suggest phosphorylation of NS5 S56 may act to shut down capping in the viral life cycle. PMID: 18757072
    13. Casein Kinase 1 phosphorylates serine 56 of yellow fever virus methyltransferase. PMID: 19185594

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  • 亚细胞定位：
    [Capsid protein C]: Virion. Host nucleus. Host cytoplasm, host perinuclear region. Host cytoplasm.; [Peptide pr]: Secreted.; [Small envelope protein M]: Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Envelope protein E]: Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Non-structural protein 1]: Secreted. Host endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side.; [Non-structural protein 2A]: Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Serine protease subunit NS2B]: Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Serine protease NS3]: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.; [Non-structural protein 4A]: Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Non-structural protein 4B]: Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [RNA-directed RNA polymerase NS5]: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Host nucleus.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, mRNA cap 0-1 NS5-type methyltransferase family
  • 数据库链接：

    KEGG: vg:1502173

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