E-Tag Monoclonal Antibody
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中文名称：
E-Tag鼠单克隆抗体
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货号：
CSB-MA000151M0m
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规格：
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图片：
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Western Blot
Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
All lanes: E-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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Western Blot
Positive WB detected in: E-tagged fusion protein
All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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Immunoprecipitating E-Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg)
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ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
Antigen at dilution of 1µg/ml
E-Tag antibody at (0-0.15µg)
Goat polyclonal to Mouse IgG at 1/50000 dilution
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Western Blot
Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
All lanes: E-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 30, 68, 55, 67-157kDa
Observed band size: 30, 68, 55, 67-157kDa
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其他：
产品详情
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产品描述：
CUSABIO货号：CSB-MA000151M0m E-Tag单克隆抗体是针对重组蛋白研究中广泛应用的E-Tag标签开发的科研工具，该标签系统常用于目标蛋白的追踪、纯化及互作研究。本产品采用高亲和力小鼠源单抗制备，能特异性识别由GAPVPYPDPLEPR氨基酸序列组成的E-Tag表位，适用于各类物种来源的E-Tag标记蛋白检测。经严格验证可在ELISA实验中实现精准定量分析，在Western Blot中清晰呈现10-150 kDa分子量范围内的靶蛋白条带，并支持免疫共沉淀（IP）技术用于蛋白相互作用研究。该抗体适用于重组蛋白表达验证、蛋白纯化过程监控以及蛋白质相互作用组学等科研场景，特别在哺乳动物表达系统、原核表达系统及体外翻译系统的研究中展现优异性能。为分子生物学、生物化学领域的E-Tag标记蛋白研究提供稳定可靠的检测支持，助力重组蛋白功能解析及信号通路机制探索。
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别名：
E-Tag
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宿主：
Mouse
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反应种属：
All
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免疫原：
GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
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标记方式：
Non-conjugated
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克隆类型：
Monoclonal Antibody
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抗体亚型：
IgG1
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纯化方式：
>95%, Protein G purified
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克隆号：
11H12B3
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, IP
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推荐稀释比：
| Application |
Recommended Dilution |
| ELISA |
1:4000-1:8000 |
| WB |
1:1000-1:320000 |
| IP |
1μg-5μg |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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