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Flag Tag Monoclonal Antibody

文献引用 (5) 说明书
  • 中文名称：
    Flag Tag鼠单克隆抗体
  • 货号：
    CSB-MA000021M0m
  • 规格：
    ￥400
  • 图片：

    • Western Blot
      Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng
      All lanes: Flag Tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng
      All lanes: Flag Tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 59.6, 36, 35, 42 kDa
      Observed band size: 59.6, 36, 35, 42 kDa

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein at 10ng
      All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng
      All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: 5 different recombinant proteins with Flag Tag
      All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution

    • Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Transfected HEK-293 cell whole cell lysate
      Lane 2: CSB-MA000021M0m (1µl) + Transfected HEK-293 cell whole cell lysate (500µg)
      Lane 3: Transfected HEK-293 cell whole cell lysate (20µg)
      For western blotting, the blot was detected with CSB-MA000021M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

    • Immunoprecipitating Flag Tag in 293F transfected whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Flag Tag in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: Company A (1µl), Company B (1µl), Company C (1µl), Company D (1µl), CSB-MA000021M0m (1µl) + Flag Tag in 293F transfected whole cell lysate (500µg)
      Lane 3: Flag Tag in 293F transfected whole cell lysate (20µg)

    • Immunofluorescence staining of 293T cells transfected with Flag tag by CSB-MA000021M0m at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without Flag tag.

  • 其他：

产品详情

  • 产品描述：
    CUSABIO货号：CSB-MA000021M0m Flag标签单克隆抗体是针对DYKDDDDK氨基酸序列开发的高特异性科研工具，该八肽标签因其亲水特性和可定向插入蛋白N端/C端的特性，已成为重组蛋白表达验证的核心分子标记。本产品采用稳定杂交瘤技术制备，具备跨物种广谱反应性，可兼容ELISA、Western Blotting、免疫荧光和免疫沉淀四大实验平台，特别适用于融合蛋白表达效率验证、亚细胞定位追踪及蛋白互作复合物捕获等研究场景。作为FLAG标签检测的关键试剂，该抗体在HEK293细胞重组表达体系验证中展现出优异的信噪比性能，配合抗体重链特异性设计有效避免轻链干扰，为基因编辑验证、信号通路蛋白互作研究提供精准的分子检测解决方案。
  • 别名：
    DDK tag, DYKDDDDK, Flag, Flag Tag
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    DYKDDDDK(Flag) synthetic peptide conjugate to KLH
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    4G5C8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:10000
    IF 1:100-1:300
    IP 0.5ul-2ul
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunofluorescence (IF) Protocol
    Immunoprecipitation (IP) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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