Myc-Tag Monoclonal Antibody
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中文名称：
Myc-Tag鼠单克隆抗体
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货号：
CSB-MA000157
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规格：
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图片：
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IP antibody use: 3µg Flag Mouse IgG1 per ml Lysate, WB 1:5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with C-Myc-tag fusion protein
Lane 3: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 4: IP (transfected 293 + anti- C-Myc mAb + Protein G agarose)
Lane 5: IP (transfected 293 + Protein G)
Lane 6: Recombinant protein (E.coli)
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IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-Myc)
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1µg Myc fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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其他：
产品详情
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产品描述：
CUSABIO货号：CSB-MA000157 Myc-Tag单克隆抗体是针对Myc表位标签研发的高特异性科研工具，采用小鼠杂交瘤技术制备的IgG型单抗，可识别重组蛋白中插入的Myc短肽序列（EQKLISEEDL）。该产品经免疫印迹、免疫荧光、免疫沉淀及ELISA多平台验证，1 mg/ml标准浓度确保实验稳定性，适用于哺乳动物、昆虫、酵母及细菌等多种表达系统的重组蛋白检测。抗体采用亲和纯化工艺制备，纯度达95%以上，冻存于含50%甘油的PBS缓冲液中，建议-20℃分装保存避免反复冻融。在蛋白质相互作用研究、亚细胞定位分析及基因工程产物验证等场景中表现优异，特别适用于融合蛋白表达效率评估、蛋白复合物共沉淀研究及荧光标记定量检测，其高亲和力特性可有效降低非特异性背景信号，是分子生物学和细胞生物学研究的关键试剂。
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宿主：
Mouse
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反应种属：
N/A
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免疫原：
Synthetic Peptide
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标记方式：
Non-conjugated
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抗体亚型：
IgG
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克隆号：
25D6
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, IF, IP
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推荐稀释比：
| Application |
Recommended Dilution |
| WB |
1:500-1:5000 |
| IF |
1:50-1:200 |
| IP |
1:200-1:2000 |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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