V5-Tag Monoclonal Antibody
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中文名称：
V5-Tag鼠单克隆抗体
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货号：
CSB-MA000160
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规格：
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图片：
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IP antibody use: 5µg V5 Mouse IgG1 per ml Lysate, WB 1: 5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with V5-tag fusion protein
Lane 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-V5 mAb + Protein G agarose)
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IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)
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1µg V5 fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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其他：
产品详情
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产品描述：
CUSABIO货号：CSB-MA000160 V5-Tag单克隆抗体是针对重组蛋白研究中广泛应用的V5表位标签开发的科研专用试剂，该表位标签源于副流感病毒血凝素神经氨酸酶蛋白，因其高特异性与低交叉反应特性成为蛋白标记及追踪实验的首选工具。本产品采用杂交瘤技术制备，经过ELISA、免疫印迹（WB）、免疫荧光（IF）和免疫沉淀（IP）等多平台验证，能精准识别带有V5标签的重组融合蛋白。其广谱物种适用性支持跨物种样本分析，适用于重组蛋白表达验证、蛋白质相互作用网络解析、亚细胞定位研究等实验体系，尤其在多组学联合分析中可配合His标签或GST标签抗体实现共表达蛋白的同步检测。该抗体经严格质控确保批次稳定性，为基因功能研究、信号通路探索及蛋白结构解析提供高灵敏度的检测解决方案，满足分子生物学、细胞生物学等领域的科研需求。
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宿主：
Mouse
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反应种属：
N/A
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免疫原：
Synthetic Peptide
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标记方式：
Non-conjugated
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抗体亚型：
IgG
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克隆号：
18G5
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, IF, IP
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推荐稀释比：
| Application |
Recommended Dilution |
| WB |
1:500-1:5000 |
| IF |
1:50-1:200 |
| IP |
1:200-1:2000 |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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