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中文名称：
His鼠单克隆抗体
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货号：
CSB-MA000011M1m
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规格：
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图片：
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Western Blot
Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4 KDa
Observed band size: 43.4 KDa
Exposure time：5min
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Western Blot
Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size:62.5 KDa
Observed band size: 66 KDa
Exposure time：5min
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Western Blot
Positive WB detected in:200ng of four Recombinant protein with his tag All lanes: His antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4, 62.5,56,80.6 KDa
Observed band size:43.4, 66, 57 ,90 KDa
Exposure time：5min
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Immunoprecipitating His in 293 whole cell lysate transfected with His
Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His. Lane 2: 2F4B5 antibody (2µg) + 293 whole cell lysate transfected with His (500µg)
Lane 3: 293 whole cell lysate transfected with His (5µg)
For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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其他：
产品详情
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产品描述：
该His Tag单克隆抗体（CUSABIO货号：CSB-MA000011M1m）专为高效检测重组蛋白中广泛应用的6×组氨酸标签设计，采用杂交瘤技术制备确保批次稳定性，在Western Blot实验中可适应1:1000至1:640,000的宽泛稀释范围，免疫沉淀实验仅需2-5μL即可有效捕获目标蛋白。该抗体具有优异的种属兼容性，经严格验证适用于ELISA、WB、IP三大核心实验体系，特别适合重组表达蛋白的定性定量分析、蛋白相互作用研究及高通量筛选等科研场景。其高亲和力特性可精准识别His标签蛋白，在变性或天然条件下均保持稳定结合能力，为蛋白质纯化工艺开发、表达验证及功能研究提供可靠工具。作为His标签检测领域的优选试剂，该产品凭借灵敏的检测限和低交叉反应性特点，广泛应用于分子生物学、结构生物学及生物制药相关的基础研究中。
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别名：
6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
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宿主：
Mouse
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反应种属：
All
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免疫原：
The immunogen is a mixture of various His-tagged proteins
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标记方式：
Non-conjugated
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克隆类型：
Monoclonal
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抗体亚型：
IgG1
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纯化方式：
>95%, Protein G purified
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克隆号：
2F4B5
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, IP
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推荐稀释比：
| Application |
Recommended Dilution |
| WB |
1:1000-1:640000 |
| IP |
2µl-5µl |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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