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  4. Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

文献引用 (15) 说明书下载
  • 中文名称：
    小鼠血管紧张素1-7（Ang1-7）酶联免疫试剂盒
  • 货号：
    CSB-E13763m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO专用于科研的小鼠血管紧张素1-7（Ang1-7）定量检测试剂盒（货号：CSB-E13763m），基于双抗体夹心酶联免疫原理，可精准检测血清、血浆、细胞培养上清及组织匀浆中的Ang1-7含量，检测灵敏度达6.25 pg/mL，线性范围为6.25-400 pg/mL。Ang1-7作为肾素-血管紧张素系统（RAS）的关键活性肽，在心血管调节、抗纤维化及炎症反应中发挥重要作用，其水平变化常被用于心血管疾病模型研究、代谢综合征机制探索及药物干预效果评估。试剂盒包含预包被捕获抗体的96孔板、梯度标准品、生物素标记检测抗体及显色底物，实验全程仅需3.5小时，操作流程标准化，支持多样本批量检测。适用于实验室对小鼠疾病模型中Ang1-7的动态监测，如高血压、心肌肥厚、肾脏损伤等研究的生物标志物分析，或体外药物筛选实验中细胞因子分泌水平的定量评估。实验需注意样本预处理，避免反复冻融，血浆样本建议使用EDTA抗凝管采集，组织样本需按比例匀浆后离心取上清检测。
  • 缩写：
    Ang1-7
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %93
    Range %87-99
    1:4Average %95
    Range %89-102
    1:8Average %95
    Range %87-98
  • 回收率：
    The recovery of mouse Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-98
    EDTA plasma (n=4)9591-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.745 2.721 2.733 2.597
    2002.316 2.245 2.281 2.145
    1001.923 1.898 1.911 1.775
    501.467 1.366 1.417 1.281
    250.796 0.788 0.792 0.656
    12.50.458 0.447 0.453 0.317
    6.250.258 0.242 0.250 0.114
    00.138 0.134 0.136 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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