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中文名称：
N重组抗体
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货号：
CSB-RA33255A1GMY
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规格：
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图片：
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Western Blot
Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
Secondary
Peroxidase-Affinipure Goat Anti-Human IgG Fc Fragment Specific at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa
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The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 1.586 ng/ml.
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In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good.
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ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.
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The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
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The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
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The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
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其他：
产品详情
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产品描述：
CSB-RA33255A1GMY N重组单克隆抗体是针对病毒核衣壳蛋白（N蛋白）研发的高特异性科研试剂，适用于病毒学基础研究与体外检测分析。该抗体采用基因重组技术制备，可精准识别N蛋白保守结构域，在ELISA、Western Blot及胶体金免疫层析（GICA）三大检测平台中均表现出优异性能。经多体系验证显示，其ELISA检测效价高达1:50000，Western Blot在1:500-1:5000稀释范围内能清晰呈现目标条带，GICA应用时推荐1:1000-1:10000梯度检测，展现良好的线性响应与批次稳定性。该产品已通过抗原表位特异性验证，对常见冠状病毒N蛋白无交叉反应，适用于病毒复制机制研究、重组蛋白表达验证、快速检测试纸条开发等科研场景，为病原体检测技术开发及抗病毒药物筛选提供可靠工具。本产品严格遵循质量管理体系生产，每批次均提供完整质检报告，确保实验数据的可重复性。
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Uniprot No.：
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别名：
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
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反应种属：
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
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免疫原：
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (
CSB-EP3325GMY)
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免疫原种属：
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
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标记方式：
Non-conjugated
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克隆类型：
Monoclonal
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抗体亚型：
Mouse scFv fusion with human IgG1 Fc
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纯化方式：
Affinity-chromatography
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克隆号：
1A6
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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产品提供形式：
Liquid
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应用范围：
ELISA, WB, GICA
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推荐稀释比：
| Application |
Recommended Dilution |
| ELISA |
1:10000-1:50000 |
| WB |
1:500-1:5000 |
| GICA |
1:1000-1:10000 |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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