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纯度：
Greater than 95% as determined by SDS-PAGE.
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内毒素：
Less than 1.0 EU/ug as determined by LAL method.
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生物活性：
①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 (CSB-MP684964PAL), the EC50 is 5.056-7.559 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 7.941-10.49 ng/ml.
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基因名：
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Uniprot No.：
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别名：
(S glycoprotein)(E2)(Peplomer protein)(Spike protein S1)(Spike protein S2)
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分子结构：
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种属：
Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
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蛋白长度：
Partial
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来源：
Mammalian cell
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分子量：
30.0 kDa
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表达区域：
306-527aa
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氨基酸序列
RVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNF
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蛋白标签：
N-terminal 10xHis-tagged and C-terminal Myc-tagged
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产品提供形式：
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
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缓冲液：
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
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复溶：
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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储存条件：
Store at -20°C/-80°C upon receipt, aliquoting is necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
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保质期：
The shelf life is related to many factors, storage state,
buffer ingredients, storage temperature and the stability of the protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
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货期：
3-7 business days
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注意事项：
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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产品描述：
Human SARS-CoV spike (S) protein amino acids 306-527 linked an N-terminal 10xHis-tag by the Thrombin linker, as well as a C-terminal Myc tag, was expressed in mammalian cells. The product is the recombinant human SARS-CoV S protein. This SARS-CoV S protein is an active protein, whose activity has been determined through functional ELISA by binding to ACE2 of Paguma larvata and human, with EC50 values of 5.056-7.559 ng/ mL and 7.941-10.49 ng/ mL, respectively. Its purity is high (>95%, SDS-PAGE) and endotoxin is low (<1.0 EU/ug protein, LAL method). Due to the glycosylation, it has an app...
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Datasheet & COA：
Please contact us to get it.
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