CANX

Western Blot
Positive WB detected in: MCF7 whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate
All lanes: CANX antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 68, 72, 56 kDa
Observed band size: 90 KDa
Exposure time：1min

Immunofluorescence staining of Hela cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunofluorescence staining of HepG2 cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Overlay Peak curve showing Hela cells stained with CSB-MA004485A1m (red line) at 1:140. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.